Death of preganglionic sympathetic neurons after surgical or immunologic lesion of peripheral processes

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Three months after systemic injection of antibody to acetylcholinesterase (AChE), there is a 60% decrease in the population of preganglionic sympathetic neurons expressing choline acetyltransferase (ChAT) in the intermediolateral (IML) nucleus of the rat spinal cord. In principle, the disappearance of identifiable cholinergic neurons might reflect either outright cell death or severe atrophy with down-regulation of cholinergic markers. To distinguish between these possibilities, preganglionic neurons were labeled with the retrograde tracer dye, Fast Blue, 1 week before antibody injection or surgical transection of the cervical sympathetic trunk. Three months after either treatment, the thoracic IML contained 40 - 60% fewer Fast Blue-labeled neurons than in controls. Therefore, preganglionic sympathetic neurons do degenerate after antibody injection or axotomy. To clarify the role of axonal damage in this process, the effects of three different mechanical lesions were examined. A lumbar ganglionectomy designed to interrupt most sympathetic axons emanating from L2 IML caused 92% loss of ChAT-positive cells observed 10 weeks later at that site. In comparison, transection of the cervical sympathetic trunk, which spared some distally directed axonal branches from the thoracic IML, caused only a 46% loss of ChAT-positive neurons at T1. Still smaller effects were seen after the same nerve was crushed, a lesion that is less destructive. Thus, the ability of central sympathetic neurons to survive a peripheral lesion may be related to the degree of axonal damage and to the opportunity for axonal regrowth.

Original languageEnglish (US)
Pages (from-to)105-114
Number of pages10
JournalExperimental Neurology
Volume177
Issue number1
DOIs
StatePublished - 2002
Externally publishedYes

Fingerprint

Neurons
Choline O-Acetyltransferase
Injections
Antibodies
Thorax
Ganglionectomy
Axotomy
Cholinergic Neurons
Population Dynamics
Acetylcholinesterase
Cholinergic Agents
Atrophy
Axons
Spinal Cord
Cell Death
Coloring Agents
Down-Regulation
diamidino compound 253-50
Therapeutics

Keywords

  • Acetylcholinesterase antibody
  • Autonomic ganglia
  • Intermediolateral nucleus
  • Nerve transection
  • Spinal cord
  • Sympathetic nervous system

ASJC Scopus subject areas

  • Neurology
  • Neuroscience(all)

Cite this

Death of preganglionic sympathetic neurons after surgical or immunologic lesion of peripheral processes. / Tang, Hui; Brimijoin, William Stephen.

In: Experimental Neurology, Vol. 177, No. 1, 2002, p. 105-114.

Research output: Contribution to journalArticle

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