Cytoskeletal modulation of sodium current in human jejunal circular smooth muscle cells

Peter R. Strege, Adrian N. Holm, Adam Rich, Steven M. Miller, Yijun Ou, Michael G. Sarr, Gianrico Farrugia

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

A Na+ current is present in human jejunal circular smooth muscle cells. The aim of the present study was to determine the role of the cytoskeleton in the regulation of the Na+ current. Whole cell currents were recorded by using standard patch-clamp techniques with Cs+ in the pipette to block K+ currents. Cytochalasin D and gelsolin were used to disrupt the actin cytoskeleton and phalloidin to stabilize it. Colchicine was used to disassemble the microtubule cytoskeleton (and intermediate filaments) and paclitaxel to stabilize it. Acrylamide was used to disrupt the intermediate filament cytoskeleton. Perfusion of the recording chamber at 10 ml/min increased peak Na+ current recorded from jejunal smooth muscle cells by 27 ± 3%. Cytochalasin D and gelsolin abolished the perfusion-induced increase in Na+ current, whereas incubation with phalloidin, colchicine, paclitaxel, or acrylamide had no effect. In conclusion, the Na+ current expressed in human jejunal circular smooth muscle cells appears to be regulated by the cytoskeleton. An intact actin cytoskeleton is required for perfusion-induced activation of the Na+ current.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume284
Issue number1 53-1
StatePublished - Jan 1 2003

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Cytoskeleton
Gelsolin
Smooth Muscle Myocytes
Muscle
Phalloidine
Cytochalasin D
Acrylamide
Sodium
Cells
Colchicine
Modulation
Paclitaxel
Intermediate Filaments
Perfusion
Actins
Actin Cytoskeleton
Clamping devices
Patch-Clamp Techniques
Chemical activation
Microtubules

Keywords

  • Actin
  • Microtubules
  • Patch clamp
  • Small intestine

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Cytoskeletal modulation of sodium current in human jejunal circular smooth muscle cells. / Strege, Peter R.; Holm, Adrian N.; Rich, Adam; Miller, Steven M.; Ou, Yijun; Sarr, Michael G.; Farrugia, Gianrico.

In: American Journal of Physiology - Cell Physiology, Vol. 284, No. 1 53-1, 01.01.2003.

Research output: Contribution to journalArticle

Strege, Peter R. ; Holm, Adrian N. ; Rich, Adam ; Miller, Steven M. ; Ou, Yijun ; Sarr, Michael G. ; Farrugia, Gianrico. / Cytoskeletal modulation of sodium current in human jejunal circular smooth muscle cells. In: American Journal of Physiology - Cell Physiology. 2003 ; Vol. 284, No. 1 53-1.
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