Cytokine production patterns and antibody response to measles vaccine

Inna G. Ovsyannikova, Karlene C. Reid, Robert M. Jacobson, Ann L Oberg, George G. Klee, Gregory A. Poland

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Cytokines play an important role in the immune response to live measles virus immunization. To gain further insight into the cytokine production profile in response to measles vaccination, we studied interferon-gamma (IFN-γ), tumor necrosis factor (TNF-α), soluble IL-2 receptor (sIL-2R), interleukin-2 (IL-2), interleukin-4 (IL-4), and interleukin-6 (IL-6) in both supernatants from peripheral blood mononuclear cells (PBMC) stimulated with phytohaemagglutinin (PHA), and plasma. We enrolled 57 healthy infants and children residing in an area where no measles virus circulated in their lifetimes. Overall analysis of cytokines in supernatants from PBMC showed that a predominant Th1 cytokine pattern occurs after the second dose of measles immunization. However, plasma levels of Th1 cytokines (IFN-γ, sIL-2R and TNF-α) were preferentially activated by measles virus after the first dose of measles vaccination. Median IFN-γ plasma levels were 1.73pg/ml for infants compared to 0.63pg/ml for older children (P=0.003). These data suggest that after the first and the second dose of measles virus immunization, there is a predominant Th1-type directed immune response, but the Th1 cytokine pattern seems to be stronger in previously unvaccinated children. There was no correlation between cytokine production by PBMC supernatants after PHA stimulation and circulating levels of plasma cytokines. No relationship was found between any specific cytokine level and measles antibody level.

Original languageEnglish (US)
Pages (from-to)3946-3953
Number of pages8
JournalVaccine
Volume21
Issue number25-26
DOIs
StatePublished - Aug 2003

Fingerprint

Measles Vaccine
Antibody Formation
cytokines
Cytokines
vaccines
Measles virus
antibodies
Measles
interleukin-2
mononuclear leukocytes
interferon-gamma
Interferon-gamma
Immunization
Blood Cells
immunization
Interleukin-2 Receptors
phytohemagglutinin
Phytohemagglutinins
Interleukin-2
Vaccination

Keywords

  • Cytokines
  • Measles
  • Vaccination

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

Cite this

Ovsyannikova, I. G., Reid, K. C., Jacobson, R. M., Oberg, A. L., Klee, G. G., & Poland, G. A. (2003). Cytokine production patterns and antibody response to measles vaccine. Vaccine, 21(25-26), 3946-3953. https://doi.org/10.1016/S0264-410X(03)00272-X

Cytokine production patterns and antibody response to measles vaccine. / Ovsyannikova, Inna G.; Reid, Karlene C.; Jacobson, Robert M.; Oberg, Ann L; Klee, George G.; Poland, Gregory A.

In: Vaccine, Vol. 21, No. 25-26, 08.2003, p. 3946-3953.

Research output: Contribution to journalArticle

Ovsyannikova, IG, Reid, KC, Jacobson, RM, Oberg, AL, Klee, GG & Poland, GA 2003, 'Cytokine production patterns and antibody response to measles vaccine', Vaccine, vol. 21, no. 25-26, pp. 3946-3953. https://doi.org/10.1016/S0264-410X(03)00272-X
Ovsyannikova IG, Reid KC, Jacobson RM, Oberg AL, Klee GG, Poland GA. Cytokine production patterns and antibody response to measles vaccine. Vaccine. 2003 Aug;21(25-26):3946-3953. https://doi.org/10.1016/S0264-410X(03)00272-X
Ovsyannikova, Inna G. ; Reid, Karlene C. ; Jacobson, Robert M. ; Oberg, Ann L ; Klee, George G. ; Poland, Gregory A. / Cytokine production patterns and antibody response to measles vaccine. In: Vaccine. 2003 ; Vol. 21, No. 25-26. pp. 3946-3953.
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