Cytokine levels in serum and synovial fluid of patients with juvenile rheumatoid arthritis

K. L. Madson, T. L. Moore, J. M. Lawrence, Thomas Osborn

Research output: Contribution to journalArticle

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Abstract

Objective. Cytokines play an important role in mediating inflammation and in regulating the immune response of many rheumatological diseases. In patients with juvenile rheumatoid arthritis (JRA), levels of 6 cytokines. interleukin 1 alpha (IL-1α), IL-1 beta (IL-1β), IL-2, IL-2 receptor (IL-2R), IL-6, and tumor necrosis factor alpha (TNF-α) were measured in serum and synovial fluid (SF) in an effort to evaluate their significance. Methods. Serum concentrations of the 6 cytokines were measured in 62 patients with JRA including 22 pauciarticular onset, 26 polyarticular onset, and 14 systemic onset patients, and 29 disease and healthy controls using enzyme-linked immunoabsorbent assays (ELISA). Seventeen SF from patients with JRA were examined for cytokine levels. Results. Elevated serum levels of IL-2R were found in patients with systemic onset and elevated IL-2 levels in pauciarticular and polyarticular onset JRA as compared to controls. Pauciarticular and polyarticular onset patients also had elevated IL-1α and IL-6 levels. There were no statistical differences found between the groups for TNF-α and IL-1β. SF revealed elevated levels of IL-1β, IL-2R, and IL-6; however, correlation was noted between serum and SF levels only for IL-1α, not for the other cytokines. Mean serum levels of IL-2R in all onset types with active disease and IL-6 levels in active polyarticular and pauciarticular onset were elevated when compared with mean inactive levels. Conclusion. Our studies indicate that (1) IL-α, IL-2, IL-2R, and IL-6 levels are increased in serum of patients with JRA with different onset types; (2) elevated levels of IL-1β, IL-2R, and IL-6 are found in their SF compared to serum levels; (3) a correlation exists between serum and SF levels only for IL-1α; (4) mean IL-2R levels are elevated with active disease in all onset types and mean IL-6 levels with active polyarticular and pauciarticular onset disease are elevated compared to mean levels of inactive patients; and (5) cytokines may thus play a role as inflammatory mediators in JRA.

Original languageEnglish (US)
Pages (from-to)2359-2363
Number of pages5
JournalJournal of Rheumatology
Volume21
Issue number12
StatePublished - Dec 1 1994
Externally publishedYes

Fingerprint

Interleukin-1alpha
Juvenile Arthritis
Synovial Fluid
Interleukin-2 Receptors
Cytokines
Interleukin-6
Serum
Interleukin-2
Tumor Necrosis Factor-alpha
Interleukin-1beta
Interleukin-1
Inflammation

Keywords

  • Cytokines
  • IL-1α
  • IL-2
  • IL-2R
  • IL-6
  • Juvenile rheumatoid arthritis

ASJC Scopus subject areas

  • Immunology
  • Rheumatology

Cite this

Cytokine levels in serum and synovial fluid of patients with juvenile rheumatoid arthritis. / Madson, K. L.; Moore, T. L.; Lawrence, J. M.; Osborn, Thomas.

In: Journal of Rheumatology, Vol. 21, No. 12, 01.12.1994, p. 2359-2363.

Research output: Contribution to journalArticle

Madson, K. L. ; Moore, T. L. ; Lawrence, J. M. ; Osborn, Thomas. / Cytokine levels in serum and synovial fluid of patients with juvenile rheumatoid arthritis. In: Journal of Rheumatology. 1994 ; Vol. 21, No. 12. pp. 2359-2363.
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abstract = "Objective. Cytokines play an important role in mediating inflammation and in regulating the immune response of many rheumatological diseases. In patients with juvenile rheumatoid arthritis (JRA), levels of 6 cytokines. interleukin 1 alpha (IL-1α), IL-1 beta (IL-1β), IL-2, IL-2 receptor (IL-2R), IL-6, and tumor necrosis factor alpha (TNF-α) were measured in serum and synovial fluid (SF) in an effort to evaluate their significance. Methods. Serum concentrations of the 6 cytokines were measured in 62 patients with JRA including 22 pauciarticular onset, 26 polyarticular onset, and 14 systemic onset patients, and 29 disease and healthy controls using enzyme-linked immunoabsorbent assays (ELISA). Seventeen SF from patients with JRA were examined for cytokine levels. Results. Elevated serum levels of IL-2R were found in patients with systemic onset and elevated IL-2 levels in pauciarticular and polyarticular onset JRA as compared to controls. Pauciarticular and polyarticular onset patients also had elevated IL-1α and IL-6 levels. There were no statistical differences found between the groups for TNF-α and IL-1β. SF revealed elevated levels of IL-1β, IL-2R, and IL-6; however, correlation was noted between serum and SF levels only for IL-1α, not for the other cytokines. Mean serum levels of IL-2R in all onset types with active disease and IL-6 levels in active polyarticular and pauciarticular onset were elevated when compared with mean inactive levels. Conclusion. Our studies indicate that (1) IL-α, IL-2, IL-2R, and IL-6 levels are increased in serum of patients with JRA with different onset types; (2) elevated levels of IL-1β, IL-2R, and IL-6 are found in their SF compared to serum levels; (3) a correlation exists between serum and SF levels only for IL-1α; (4) mean IL-2R levels are elevated with active disease in all onset types and mean IL-6 levels with active polyarticular and pauciarticular onset disease are elevated compared to mean levels of inactive patients; and (5) cytokines may thus play a role as inflammatory mediators in JRA.",
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T1 - Cytokine levels in serum and synovial fluid of patients with juvenile rheumatoid arthritis

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AU - Moore, T. L.

AU - Lawrence, J. M.

AU - Osborn, Thomas

PY - 1994/12/1

Y1 - 1994/12/1

N2 - Objective. Cytokines play an important role in mediating inflammation and in regulating the immune response of many rheumatological diseases. In patients with juvenile rheumatoid arthritis (JRA), levels of 6 cytokines. interleukin 1 alpha (IL-1α), IL-1 beta (IL-1β), IL-2, IL-2 receptor (IL-2R), IL-6, and tumor necrosis factor alpha (TNF-α) were measured in serum and synovial fluid (SF) in an effort to evaluate their significance. Methods. Serum concentrations of the 6 cytokines were measured in 62 patients with JRA including 22 pauciarticular onset, 26 polyarticular onset, and 14 systemic onset patients, and 29 disease and healthy controls using enzyme-linked immunoabsorbent assays (ELISA). Seventeen SF from patients with JRA were examined for cytokine levels. Results. Elevated serum levels of IL-2R were found in patients with systemic onset and elevated IL-2 levels in pauciarticular and polyarticular onset JRA as compared to controls. Pauciarticular and polyarticular onset patients also had elevated IL-1α and IL-6 levels. There were no statistical differences found between the groups for TNF-α and IL-1β. SF revealed elevated levels of IL-1β, IL-2R, and IL-6; however, correlation was noted between serum and SF levels only for IL-1α, not for the other cytokines. Mean serum levels of IL-2R in all onset types with active disease and IL-6 levels in active polyarticular and pauciarticular onset were elevated when compared with mean inactive levels. Conclusion. Our studies indicate that (1) IL-α, IL-2, IL-2R, and IL-6 levels are increased in serum of patients with JRA with different onset types; (2) elevated levels of IL-1β, IL-2R, and IL-6 are found in their SF compared to serum levels; (3) a correlation exists between serum and SF levels only for IL-1α; (4) mean IL-2R levels are elevated with active disease in all onset types and mean IL-6 levels with active polyarticular and pauciarticular onset disease are elevated compared to mean levels of inactive patients; and (5) cytokines may thus play a role as inflammatory mediators in JRA.

AB - Objective. Cytokines play an important role in mediating inflammation and in regulating the immune response of many rheumatological diseases. In patients with juvenile rheumatoid arthritis (JRA), levels of 6 cytokines. interleukin 1 alpha (IL-1α), IL-1 beta (IL-1β), IL-2, IL-2 receptor (IL-2R), IL-6, and tumor necrosis factor alpha (TNF-α) were measured in serum and synovial fluid (SF) in an effort to evaluate their significance. Methods. Serum concentrations of the 6 cytokines were measured in 62 patients with JRA including 22 pauciarticular onset, 26 polyarticular onset, and 14 systemic onset patients, and 29 disease and healthy controls using enzyme-linked immunoabsorbent assays (ELISA). Seventeen SF from patients with JRA were examined for cytokine levels. Results. Elevated serum levels of IL-2R were found in patients with systemic onset and elevated IL-2 levels in pauciarticular and polyarticular onset JRA as compared to controls. Pauciarticular and polyarticular onset patients also had elevated IL-1α and IL-6 levels. There were no statistical differences found between the groups for TNF-α and IL-1β. SF revealed elevated levels of IL-1β, IL-2R, and IL-6; however, correlation was noted between serum and SF levels only for IL-1α, not for the other cytokines. Mean serum levels of IL-2R in all onset types with active disease and IL-6 levels in active polyarticular and pauciarticular onset were elevated when compared with mean inactive levels. Conclusion. Our studies indicate that (1) IL-α, IL-2, IL-2R, and IL-6 levels are increased in serum of patients with JRA with different onset types; (2) elevated levels of IL-1β, IL-2R, and IL-6 are found in their SF compared to serum levels; (3) a correlation exists between serum and SF levels only for IL-1α; (4) mean IL-2R levels are elevated with active disease in all onset types and mean IL-6 levels with active polyarticular and pauciarticular onset disease are elevated compared to mean levels of inactive patients; and (5) cytokines may thus play a role as inflammatory mediators in JRA.

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