Cytokine and chemokine profiles in multiple myeloma; significance of stromal interaction and correlation of IL-8 production with disease progression

Michael Kline, Kathleen Donovan, Linda Wellik, Christopher Lust, Wendy Jin, Laurie Moon-Tasson, Yuning Xiong, Thomas Elmer Witzig, Shaji K Kumar, S Vincent Rajkumar, John A. Lust

Research output: Contribution to journalArticle

51 Scopus citations

Abstract

Multiple myeloma (MM) is a product of interactions between tumor plasma cells and multiple cell types native to the bone marrow (BM). We have used antibody array technology to examine the proteins produced by BM stromal cells in response to stimulation by BM taken from patients diagnosed with monoclonal gammopathy of undetermined significance (MGUS), smoldering multiple myeloma (SMM), and MM. We observed increased production of the chemokine IL-8 by stromal cells co-cultured with supernatants from bone marrow cells of patients with active myeloma. IL-8 production is correlated with active disease and is dependent upon IL-1β and NF-κB signaling. Consistent with the pro-angiogenic activity of IL-8, increased BM microvessel density (MVD) correlated with stimulation of stromal cell IL-8 production. In addition, the majority of MM cell lines and MM patient plasma cells were found to express IL-8 receptors CXCR1 and CXCR2. We conclude that stromal cell IL-8 production parallels MM disease activity, is IL-1β induced, and correlates with bone marrow angiogenesis.

Original languageEnglish (US)
Pages (from-to)591-598
Number of pages8
JournalLeukemia Research
Volume31
Issue number5
DOIs
StatePublished - May 2007

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Keywords

  • Angiogenesis
  • Arrays
  • Chemokines
  • Cytokines
  • Multiple myeloma

ASJC Scopus subject areas

  • Cancer Research
  • Hematology
  • Oncology

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