TY - JOUR
T1 - Cytogenetic methods for the mouse
T2 - Preparation of chromosomes, karyotyping, and in situ hybridization
AU - Lee, James J.
AU - Warburton, Dorothy
AU - Robertson, Elizabeth J.
N1 - Funding Information:
We thank Edward P. Evans for providing the data appearing in Fig. 3. His generosity and expertise have enriched the quality of this manuscript immeasurably. We thank Drew Olsen for his assistance in modifying many of the protocols, and Vassilis Pachnis for making data available to us (Fig. 4b). We also thank Frank Costantini, Debra Wolgemuth, and Nancy Lee for their critical review of this manuscript. Work by J.J.L. was performed in the laboratory of Frank Cos-tantini, supported in part by an ACS postdoctoral fellowship (Grant 03028).
PY - 1990/8/15
Y1 - 1990/8/15
N2 - The ability to examine the chromosomes of murine cells is rapidly becoming essential to the mouse molecular geneticist. Although these technologies exist, the protocols are scattered throughout the literature, and in some cases different protocols exist for the same procedure. We attempt here both to augment and to consolidate this body of work. This review provides a complete set of procedures for manipulating mouse chromosomes. Prospective sources of metaphase chromosomes are discussed and detailed protocols are outlined for their preparation and characterization. We assume that the reader has little or no cytogenetic background and have included a troubleshooting guide to assist the process. Protocols for in situ hybridization to metaphase spreads that allow the rapid localization of sequences using nonradioactive probes are also included. Variations in the protocols described here abound and alternatives are indicated.
AB - The ability to examine the chromosomes of murine cells is rapidly becoming essential to the mouse molecular geneticist. Although these technologies exist, the protocols are scattered throughout the literature, and in some cases different protocols exist for the same procedure. We attempt here both to augment and to consolidate this body of work. This review provides a complete set of procedures for manipulating mouse chromosomes. Prospective sources of metaphase chromosomes are discussed and detailed protocols are outlined for their preparation and characterization. We assume that the reader has little or no cytogenetic background and have included a troubleshooting guide to assist the process. Protocols for in situ hybridization to metaphase spreads that allow the rapid localization of sequences using nonradioactive probes are also included. Variations in the protocols described here abound and alternatives are indicated.
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U2 - 10.1016/0003-2697(90)90036-9
DO - 10.1016/0003-2697(90)90036-9
M3 - Review article
C2 - 2278379
AN - SCOPUS:0025051032
SN - 0003-2697
VL - 189
SP - 1
EP - 17
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 1
ER -