Cytogenetic and fluorescence in situ hybridization studies on sporadic and hereditary tumors associated with von Hippel-Lindau syndrome (VHL)

Hans Joachim H. Decker, Sabine M. Klauck, Jeanne B. Lawrence, John McNeil, David Smith, Robert M. Gemmill, Avery A. Sandberg, Hartmut H.P. Neumann, Babette Simon, Jane Green, Bernd R. Seizinger

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

We performed cytogenetic and fluorescence in situ hybridization (FISH) studies on 29 sporadic or familial tumors associated with von Hippel-Landau disease. Four of five renal cell carcinomas with detectable alterations showed clones with chromosome 3 alterations. These changes led to loss of genetic material visible with cytogenetic resolution: either an unbalanced translocation involving 3p or loss of a whole homolog 3, resulting in monosomy of 3p. We have previously mapped the VHL gene to chromosomal region 3p25-p26. We applied FISH using the single copy probes cA233 and cA479, sequences close to the VHL gene, in a search for submicroscopic deletions of 3p. Use of FISH with differentially labeled probes indicated cA479 to be distal to cA233, but both were located within bands 3p25-26. FISH with single copy probes for interphase cytogenetics detected four subclones with deletions in the VHL region in 8 22 tumors, including four tumors which appeared cytogenetically normal. FISH proved to be a powerful tool in tumor genetic studies, especially helpful in detecting tumor subclones in benign and slowly growing tumors.

Original languageEnglish (US)
Pages (from-to)1-13
Number of pages13
JournalCancer Genetics and Cytogenetics
Volume77
Issue number1
DOIs
StatePublished - Oct 1 1994

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

Fingerprint Dive into the research topics of 'Cytogenetic and fluorescence in situ hybridization studies on sporadic and hereditary tumors associated with von Hippel-Lindau syndrome (VHL)'. Together they form a unique fingerprint.

  • Cite this

    Decker, H. J. H., Klauck, S. M., Lawrence, J. B., McNeil, J., Smith, D., Gemmill, R. M., Sandberg, A. A., Neumann, H. H. P., Simon, B., Green, J., & Seizinger, B. R. (1994). Cytogenetic and fluorescence in situ hybridization studies on sporadic and hereditary tumors associated with von Hippel-Lindau syndrome (VHL). Cancer Genetics and Cytogenetics, 77(1), 1-13. https://doi.org/10.1016/0165-4608(94)90141-4