Cytochrome P450 and glutathione transferase expression in human breast cancer

Basil F. El-Rayes, Shadan Ali, Lance K. Heilbrun, Samir Lababidi, David Bouwman, Daniel W Visscher, Philip A. Philip

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Purpose: The cytochrome P-450 (CYP) and glutathione S-transferase (GST) enzyme systems may influence the biological effects of carcinogens, including estrogens. As such, these enzymes may predict the developmental risk of breast cancer, as well as be potential targets for chemoprevention. The purpose of this study was to compare the expression of GST-Pi and CYPs 1A1, 2B6, 2E1, and 3A4 in paired samples of normal and malignant breast tissue from patients with breast cancer and women undergoing reduction mammoplasty. Experimental design: Expression of CYPs 1A1, 2B6, 2E1, 3A4, and GST-Pi was quantified in breast tissue from 33 patients with breast cancer and in 17 women without history of cancer who underwent reduction mammoplasty. The expression of CYP 1A1, 2B6, 2E1, 3A4, and GST-Pi was quantified by immunoblotting. Results: CYP 1A1, 2E1, and 3A4 expression was significantly lower (P <0.05) in malignant tissue as compared with morphologically normal adjacent tissue. Conversely, GST-Pi expression was marginally lower in the normal tissue (P = 0.08). No significant difference in enzyme expression was seen between the tissue from reduction mammoplasty and normal tissue from breast cancer patients. There was a trend for higher expression of CYP 2B6 and GST-Pi in the estrogen receptor expressing tumors than those tumors without expression (P > 0.28). Conclusion: The expression of these enzymes was similar in morphologically normal breast tissue from patients with or without breast cancer. The expression of CYPs was down-regulated in the tumor tissue. The clinical significance of CYP alterations in breast cancer will need further characterization.

Original languageEnglish (US)
Pages (from-to)1705-1709
Number of pages5
JournalClinical Cancer Research
Volume9
Issue number5
StatePublished - May 1 2003
Externally publishedYes

Fingerprint

Glutathione Transferase
Cytochrome P-450 Enzyme System
Glutathione S-Transferase pi
Breast Neoplasms
Cytochrome P-450 CYP1A1
Breast
Mammaplasty
Enzymes
Chemoprevention
Immunoblotting
Carcinogens
Neoplasms
Estrogens
Research Design

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

El-Rayes, B. F., Ali, S., Heilbrun, L. K., Lababidi, S., Bouwman, D., Visscher, D. W., & Philip, P. A. (2003). Cytochrome P450 and glutathione transferase expression in human breast cancer. Clinical Cancer Research, 9(5), 1705-1709.

Cytochrome P450 and glutathione transferase expression in human breast cancer. / El-Rayes, Basil F.; Ali, Shadan; Heilbrun, Lance K.; Lababidi, Samir; Bouwman, David; Visscher, Daniel W; Philip, Philip A.

In: Clinical Cancer Research, Vol. 9, No. 5, 01.05.2003, p. 1705-1709.

Research output: Contribution to journalArticle

El-Rayes, BF, Ali, S, Heilbrun, LK, Lababidi, S, Bouwman, D, Visscher, DW & Philip, PA 2003, 'Cytochrome P450 and glutathione transferase expression in human breast cancer', Clinical Cancer Research, vol. 9, no. 5, pp. 1705-1709.
El-Rayes BF, Ali S, Heilbrun LK, Lababidi S, Bouwman D, Visscher DW et al. Cytochrome P450 and glutathione transferase expression in human breast cancer. Clinical Cancer Research. 2003 May 1;9(5):1705-1709.
El-Rayes, Basil F. ; Ali, Shadan ; Heilbrun, Lance K. ; Lababidi, Samir ; Bouwman, David ; Visscher, Daniel W ; Philip, Philip A. / Cytochrome P450 and glutathione transferase expression in human breast cancer. In: Clinical Cancer Research. 2003 ; Vol. 9, No. 5. pp. 1705-1709.
@article{bafaf1889e5a44c9b0d12efe19ff5e58,
title = "Cytochrome P450 and glutathione transferase expression in human breast cancer",
abstract = "Purpose: The cytochrome P-450 (CYP) and glutathione S-transferase (GST) enzyme systems may influence the biological effects of carcinogens, including estrogens. As such, these enzymes may predict the developmental risk of breast cancer, as well as be potential targets for chemoprevention. The purpose of this study was to compare the expression of GST-Pi and CYPs 1A1, 2B6, 2E1, and 3A4 in paired samples of normal and malignant breast tissue from patients with breast cancer and women undergoing reduction mammoplasty. Experimental design: Expression of CYPs 1A1, 2B6, 2E1, 3A4, and GST-Pi was quantified in breast tissue from 33 patients with breast cancer and in 17 women without history of cancer who underwent reduction mammoplasty. The expression of CYP 1A1, 2B6, 2E1, 3A4, and GST-Pi was quantified by immunoblotting. Results: CYP 1A1, 2E1, and 3A4 expression was significantly lower (P <0.05) in malignant tissue as compared with morphologically normal adjacent tissue. Conversely, GST-Pi expression was marginally lower in the normal tissue (P = 0.08). No significant difference in enzyme expression was seen between the tissue from reduction mammoplasty and normal tissue from breast cancer patients. There was a trend for higher expression of CYP 2B6 and GST-Pi in the estrogen receptor expressing tumors than those tumors without expression (P > 0.28). Conclusion: The expression of these enzymes was similar in morphologically normal breast tissue from patients with or without breast cancer. The expression of CYPs was down-regulated in the tumor tissue. The clinical significance of CYP alterations in breast cancer will need further characterization.",
author = "El-Rayes, {Basil F.} and Shadan Ali and Heilbrun, {Lance K.} and Samir Lababidi and David Bouwman and Visscher, {Daniel W} and Philip, {Philip A.}",
year = "2003",
month = "5",
day = "1",
language = "English (US)",
volume = "9",
pages = "1705--1709",
journal = "Clinical Cancer Research",
issn = "1078-0432",
publisher = "American Association for Cancer Research Inc.",
number = "5",

}

TY - JOUR

T1 - Cytochrome P450 and glutathione transferase expression in human breast cancer

AU - El-Rayes, Basil F.

AU - Ali, Shadan

AU - Heilbrun, Lance K.

AU - Lababidi, Samir

AU - Bouwman, David

AU - Visscher, Daniel W

AU - Philip, Philip A.

PY - 2003/5/1

Y1 - 2003/5/1

N2 - Purpose: The cytochrome P-450 (CYP) and glutathione S-transferase (GST) enzyme systems may influence the biological effects of carcinogens, including estrogens. As such, these enzymes may predict the developmental risk of breast cancer, as well as be potential targets for chemoprevention. The purpose of this study was to compare the expression of GST-Pi and CYPs 1A1, 2B6, 2E1, and 3A4 in paired samples of normal and malignant breast tissue from patients with breast cancer and women undergoing reduction mammoplasty. Experimental design: Expression of CYPs 1A1, 2B6, 2E1, 3A4, and GST-Pi was quantified in breast tissue from 33 patients with breast cancer and in 17 women without history of cancer who underwent reduction mammoplasty. The expression of CYP 1A1, 2B6, 2E1, 3A4, and GST-Pi was quantified by immunoblotting. Results: CYP 1A1, 2E1, and 3A4 expression was significantly lower (P <0.05) in malignant tissue as compared with morphologically normal adjacent tissue. Conversely, GST-Pi expression was marginally lower in the normal tissue (P = 0.08). No significant difference in enzyme expression was seen between the tissue from reduction mammoplasty and normal tissue from breast cancer patients. There was a trend for higher expression of CYP 2B6 and GST-Pi in the estrogen receptor expressing tumors than those tumors without expression (P > 0.28). Conclusion: The expression of these enzymes was similar in morphologically normal breast tissue from patients with or without breast cancer. The expression of CYPs was down-regulated in the tumor tissue. The clinical significance of CYP alterations in breast cancer will need further characterization.

AB - Purpose: The cytochrome P-450 (CYP) and glutathione S-transferase (GST) enzyme systems may influence the biological effects of carcinogens, including estrogens. As such, these enzymes may predict the developmental risk of breast cancer, as well as be potential targets for chemoprevention. The purpose of this study was to compare the expression of GST-Pi and CYPs 1A1, 2B6, 2E1, and 3A4 in paired samples of normal and malignant breast tissue from patients with breast cancer and women undergoing reduction mammoplasty. Experimental design: Expression of CYPs 1A1, 2B6, 2E1, 3A4, and GST-Pi was quantified in breast tissue from 33 patients with breast cancer and in 17 women without history of cancer who underwent reduction mammoplasty. The expression of CYP 1A1, 2B6, 2E1, 3A4, and GST-Pi was quantified by immunoblotting. Results: CYP 1A1, 2E1, and 3A4 expression was significantly lower (P <0.05) in malignant tissue as compared with morphologically normal adjacent tissue. Conversely, GST-Pi expression was marginally lower in the normal tissue (P = 0.08). No significant difference in enzyme expression was seen between the tissue from reduction mammoplasty and normal tissue from breast cancer patients. There was a trend for higher expression of CYP 2B6 and GST-Pi in the estrogen receptor expressing tumors than those tumors without expression (P > 0.28). Conclusion: The expression of these enzymes was similar in morphologically normal breast tissue from patients with or without breast cancer. The expression of CYPs was down-regulated in the tumor tissue. The clinical significance of CYP alterations in breast cancer will need further characterization.

UR - http://www.scopus.com/inward/record.url?scp=0037652178&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037652178&partnerID=8YFLogxK

M3 - Article

VL - 9

SP - 1705

EP - 1709

JO - Clinical Cancer Research

JF - Clinical Cancer Research

SN - 1078-0432

IS - 5

ER -