Cysteines flanking the internal fusion peptide are required for the avian sarcoma/leukosis virus glycoprotein to mediate the lipid mixing stage of fusion with high efficiency

Sue E. Delos, Matthew B. Brecher, Zaoying Chen, Deborah C. Melder, Mark J Federspiel, Judith M. White

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

We previously showed that the cysteines flanking the internal fusion peptide of the avian sarcoma/leukosis virus subtype A (ASLV-A) Env (EnvA) are important for infectivity and cell-cell fusion. Here we define the stage of fusion at which the cysteines are required. The flanking cysteines are dispensable for receptor-triggered membrane association but are required for the lipid mixing step of fusion, which, interestingly, displays a high pH onset and a biphasic profile. Second-site mutations that partially restore infection partially restore lipid mixing. These findings indicate that the cysteines flanking the internal fusion peptide of EnvA (and perhaps by analogy Ebola virus glycoprotein) are important for the foldback stage of the conformational changes that lead to membrane merger.

Original languageEnglish (US)
Pages (from-to)3131-3134
Number of pages4
JournalJournal of Virology
Volume82
Issue number6
DOIs
StatePublished - Mar 2008

Fingerprint

avian sarcoma
Alpharetrovirus
Cysteine
cysteine
glycoproteins
Glycoproteins
peptides
Lipids
viruses
Peptides
lipids
Ebolavirus
cell fusion
Membranes
Cell Fusion
pathogenicity
mutation
Mutation
receptors
Infection

ASJC Scopus subject areas

  • Immunology

Cite this

Cysteines flanking the internal fusion peptide are required for the avian sarcoma/leukosis virus glycoprotein to mediate the lipid mixing stage of fusion with high efficiency. / Delos, Sue E.; Brecher, Matthew B.; Chen, Zaoying; Melder, Deborah C.; Federspiel, Mark J; White, Judith M.

In: Journal of Virology, Vol. 82, No. 6, 03.2008, p. 3131-3134.

Research output: Contribution to journalArticle

@article{19badad5ff7c4c06890f1ce38e2c0553,
title = "Cysteines flanking the internal fusion peptide are required for the avian sarcoma/leukosis virus glycoprotein to mediate the lipid mixing stage of fusion with high efficiency",
abstract = "We previously showed that the cysteines flanking the internal fusion peptide of the avian sarcoma/leukosis virus subtype A (ASLV-A) Env (EnvA) are important for infectivity and cell-cell fusion. Here we define the stage of fusion at which the cysteines are required. The flanking cysteines are dispensable for receptor-triggered membrane association but are required for the lipid mixing step of fusion, which, interestingly, displays a high pH onset and a biphasic profile. Second-site mutations that partially restore infection partially restore lipid mixing. These findings indicate that the cysteines flanking the internal fusion peptide of EnvA (and perhaps by analogy Ebola virus glycoprotein) are important for the foldback stage of the conformational changes that lead to membrane merger.",
author = "Delos, {Sue E.} and Brecher, {Matthew B.} and Zaoying Chen and Melder, {Deborah C.} and Federspiel, {Mark J} and White, {Judith M.}",
year = "2008",
month = "3",
doi = "10.1128/JVI.02266-07",
language = "English (US)",
volume = "82",
pages = "3131--3134",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "6",

}

TY - JOUR

T1 - Cysteines flanking the internal fusion peptide are required for the avian sarcoma/leukosis virus glycoprotein to mediate the lipid mixing stage of fusion with high efficiency

AU - Delos, Sue E.

AU - Brecher, Matthew B.

AU - Chen, Zaoying

AU - Melder, Deborah C.

AU - Federspiel, Mark J

AU - White, Judith M.

PY - 2008/3

Y1 - 2008/3

N2 - We previously showed that the cysteines flanking the internal fusion peptide of the avian sarcoma/leukosis virus subtype A (ASLV-A) Env (EnvA) are important for infectivity and cell-cell fusion. Here we define the stage of fusion at which the cysteines are required. The flanking cysteines are dispensable for receptor-triggered membrane association but are required for the lipid mixing step of fusion, which, interestingly, displays a high pH onset and a biphasic profile. Second-site mutations that partially restore infection partially restore lipid mixing. These findings indicate that the cysteines flanking the internal fusion peptide of EnvA (and perhaps by analogy Ebola virus glycoprotein) are important for the foldback stage of the conformational changes that lead to membrane merger.

AB - We previously showed that the cysteines flanking the internal fusion peptide of the avian sarcoma/leukosis virus subtype A (ASLV-A) Env (EnvA) are important for infectivity and cell-cell fusion. Here we define the stage of fusion at which the cysteines are required. The flanking cysteines are dispensable for receptor-triggered membrane association but are required for the lipid mixing step of fusion, which, interestingly, displays a high pH onset and a biphasic profile. Second-site mutations that partially restore infection partially restore lipid mixing. These findings indicate that the cysteines flanking the internal fusion peptide of EnvA (and perhaps by analogy Ebola virus glycoprotein) are important for the foldback stage of the conformational changes that lead to membrane merger.

UR - http://www.scopus.com/inward/record.url?scp=40149083415&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=40149083415&partnerID=8YFLogxK

U2 - 10.1128/JVI.02266-07

DO - 10.1128/JVI.02266-07

M3 - Article

VL - 82

SP - 3131

EP - 3134

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 6

ER -