Cysteine-rich secretory protein-3: A potential biomarker for prostate cancer

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Abstract

Electronic profiling of publicly available expressed sequence tag databases identified a gene, cysteine-rich secretory protein-3 (CRISP-3), that is up-regulated in prostate cancer, and of which the expression is relatively prostate-specific. The objective of this study was to examine the potential of CRISP-3 as a biomarker for prostate cancer. In transient transfection studies, CRISP- 3 was found to be a secretory protein. Using a multiple tissue dot blot experiment, CRISP-3 transcript was identified in a limited number of human tissues including the prostate. In situ hybridization experiments indicated that CRISP-3 mRNA is epithelial-specific and is upregulated in prostate adenocarcinoma compared with benign prostate tissue. CRISP-3 mRNA overexpression in cancer was confirmed using quantitative real-time reverse-transcription-PCR using benign prostatic epithelia and adenocarcinoma (in 5 of 5 cases) isolated by laser capture microdissection, as well as bulk tissues (in 20 of 23 cases) from surgically resected human prostates. These findings suggest that CRISP-3 is a potential biomarker for prostate cancer.

Original languageEnglish (US)
Pages (from-to)1419-1426
Number of pages8
JournalCancer Epidemiology Biomarkers and Prevention
Volume11
Issue number11
StatePublished - Nov 1 2002

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ASJC Scopus subject areas

  • Epidemiology
  • Oncology

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