Cyclosporine-induced renal artery smooth muscle contraction is associated with increases in the phosphorylation of specific contractile regulatory proteins

Arthur Beall, Aaron Epstein, David Woodrum, Colleen M. Brophy

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Cyclosporine A (CSA) is a type 2B phosphatase inhibitor which can induce contraction of renal artery smooth muscle. In this investigation, we examined the phosphorylation events associated with CSA-induced contraction of bovine renal artery smooth muscle. Contractile responses were determined in a muscle bath and the corresponding phosphorylation events were determined with whole cell phosphorylation and two-dimensional gel electrophoresis. CSA-induced contractions were associated with increases in the phosphorylation of the 20 kDa myosin light chains (MLC20) and different isoforms of the small heat shock protein, HSP27. Cyclic nucleotide-dependent relaxation of CSA-induced contractions was associated with increases in the phosphorylation of another small heat shock protein, HSP20, and decreases in the phosphorylation of the MLC20, and some isoforms of HSP27. These data suggest that CSA-induced contraction and relaxation of vascular smooth muscle is associated with increases in the phosphorylation of specific contractile regulatory proteins. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)41-49
Number of pages9
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume1449
Issue number1
DOIs
StatePublished - Feb 9 1999

Keywords

  • Cyclosporine
  • Heat shock protein
  • Myosin light chain
  • Smooth muscle, vascular

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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