Cycloheximide inhibits the induction of collagenase mRNA in chondrocytes exposed to synovial factors or recombinant interleukin-1

C. W. Lin, H. I. Georgescu, S. L. Phillips, C. H. Evans

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Synoviocytes secrete "chondrocyte activating factors" (CAF) which, like recombinant interleukin-1 (IL-1), induce the synthesis of collagenase by cultures of articular chondrocytes. Enzyme synthesis is preceded by the appearance of collagenase mRNA some 3-5 hours after exposure of the chondrocytes to CAF or IL-1. Cycloheximide (CX) inhibits the appearance of this message in a dose-dependent manner. At a concentration of 5 μg/ml inhibition by CX is completely reversible, with superinduction being observed in certain experiments. Identification of the newly synthesised proteins which are required for collagenase mRNA induction would greatly advance our understanding of collagenase gene expression

Original languageEnglish (US)
Pages (from-to)445-447
Number of pages3
JournalAgents and Actions
Volume27
Issue number3-4
DOIs
StatePublished - Jun 1 1989

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ASJC Scopus subject areas

  • Toxicology
  • Pharmacology (medical)

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