Cyclin D1 promotes anchorage-independent cell survival by inhibiting FOXO-mediated anoikis

L. Gan; P. Liu; S. Chen; J. Yang; J. B. McCarthy; K. E. Knudsen; H. Huang

doi:10.1038/cdd.2009.86

Cyclin D1 promotes anchorage-independent cell survival by inhibiting FOXO-mediated anoikis

L. Gan, P. Liu, S. Chen, J. Yang, J. B. McCarthy, K. E. Knudsen, H. Huang

Biochemistry and Molecular Biology

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

O-class forkhead box (FOXO) transcription factors are critical regulators of diverse cellular processes, including apoptosis, cellcycle arrest, DNA damage repair and oxidative stress resistance. Here, we show that FOXO1 and FOXO3a have an essential function in promoting cell detachment-induced anoikis, resistance to which is implicated in cancer development and metastasis. In contrast, the oncoprotein cyclin D1 inhibits anoikis. We further show that cyclin D1 interacts with FOXO proteins and impedes their transcriptional regulatory and anoikis-promoting functions. This effect of cyclin D1 requires its transcription repression domain but is independent of cyclin-dependent kinases CDK4 and CDK6. Moreover, we show that cancer-derived mutants of cyclin D1 are much more stable than wild-type cyclin D1 under anchorage-independent conditions and possess a greater antagonistic effect on FOXO-regulated anoikis and anchorage-independent growth of cancer cells. These data suggest that cyclin D1 may have a critical function in tumorigenesis and cancer metastasis by inhibiting the anoikis-promoting function of FOXO proteins.

Original language	English (US)
Pages (from-to)	1408-1417
Number of pages	10
Journal	Cell Death and Differentiation
Volume	16
Issue number	10
DOIs	https://doi.org/10.1038/cdd.2009.86
State	Published - 2009

ASJC Scopus subject areas

Molecular Biology
Cell Biology

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10.1038/cdd.2009.86

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@article{e44e429a9fe945e1b23aabd11e04dbf1,

title = "Cyclin D1 promotes anchorage-independent cell survival by inhibiting FOXO-mediated anoikis",

abstract = "O-class forkhead box (FOXO) transcription factors are critical regulators of diverse cellular processes, including apoptosis, cellcycle arrest, DNA damage repair and oxidative stress resistance. Here, we show that FOXO1 and FOXO3a have an essential function in promoting cell detachment-induced anoikis, resistance to which is implicated in cancer development and metastasis. In contrast, the oncoprotein cyclin D1 inhibits anoikis. We further show that cyclin D1 interacts with FOXO proteins and impedes their transcriptional regulatory and anoikis-promoting functions. This effect of cyclin D1 requires its transcription repression domain but is independent of cyclin-dependent kinases CDK4 and CDK6. Moreover, we show that cancer-derived mutants of cyclin D1 are much more stable than wild-type cyclin D1 under anchorage-independent conditions and possess a greater antagonistic effect on FOXO-regulated anoikis and anchorage-independent growth of cancer cells. These data suggest that cyclin D1 may have a critical function in tumorigenesis and cancer metastasis by inhibiting the anoikis-promoting function of FOXO proteins.",

author = "L. Gan and P. Liu and S. Chen and J. Yang and McCarthy, {J. B.} and Knudsen, {K. E.} and H. Huang",

note = "Funding Information: Acknowledgements. We thank DJ Tindall, KL Guan, M Pagano, CY Young, and SW Hayward for plasmids and cell lines, Y Shimizu, V Bardwell, KL Schwertfeger, SM Dehm and members of Huang laboratory, especially LR Bohrer for critical reading of the manuscript. This work was supported in part by funds from the Department of Defense (W81XWH-07-1-0137 and PC080591) and the Brainstorm Award from the University of Minnesota Masonic Cancer Center (to HH), the National Institutes of Health (CA82295 to JBM and CA099996 to KEK), and the National Natural Science Foundation of China (30500109 to LG). All authors claim no conflict of interest with this study.",

year = "2009",

doi = "10.1038/cdd.2009.86",

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AU - Gan, L.

AU - Liu, P.

AU - Chen, S.

AU - Yang, J.

AU - McCarthy, J. B.

AU - Knudsen, K. E.

AU - Huang, H.

N1 - Funding Information: Acknowledgements. We thank DJ Tindall, KL Guan, M Pagano, CY Young, and SW Hayward for plasmids and cell lines, Y Shimizu, V Bardwell, KL Schwertfeger, SM Dehm and members of Huang laboratory, especially LR Bohrer for critical reading of the manuscript. This work was supported in part by funds from the Department of Defense (W81XWH-07-1-0137 and PC080591) and the Brainstorm Award from the University of Minnesota Masonic Cancer Center (to HH), the National Institutes of Health (CA82295 to JBM and CA099996 to KEK), and the National Natural Science Foundation of China (30500109 to LG). All authors claim no conflict of interest with this study.

PY - 2009

Y1 - 2009

N2 - O-class forkhead box (FOXO) transcription factors are critical regulators of diverse cellular processes, including apoptosis, cellcycle arrest, DNA damage repair and oxidative stress resistance. Here, we show that FOXO1 and FOXO3a have an essential function in promoting cell detachment-induced anoikis, resistance to which is implicated in cancer development and metastasis. In contrast, the oncoprotein cyclin D1 inhibits anoikis. We further show that cyclin D1 interacts with FOXO proteins and impedes their transcriptional regulatory and anoikis-promoting functions. This effect of cyclin D1 requires its transcription repression domain but is independent of cyclin-dependent kinases CDK4 and CDK6. Moreover, we show that cancer-derived mutants of cyclin D1 are much more stable than wild-type cyclin D1 under anchorage-independent conditions and possess a greater antagonistic effect on FOXO-regulated anoikis and anchorage-independent growth of cancer cells. These data suggest that cyclin D1 may have a critical function in tumorigenesis and cancer metastasis by inhibiting the anoikis-promoting function of FOXO proteins.

AB - O-class forkhead box (FOXO) transcription factors are critical regulators of diverse cellular processes, including apoptosis, cellcycle arrest, DNA damage repair and oxidative stress resistance. Here, we show that FOXO1 and FOXO3a have an essential function in promoting cell detachment-induced anoikis, resistance to which is implicated in cancer development and metastasis. In contrast, the oncoprotein cyclin D1 inhibits anoikis. We further show that cyclin D1 interacts with FOXO proteins and impedes their transcriptional regulatory and anoikis-promoting functions. This effect of cyclin D1 requires its transcription repression domain but is independent of cyclin-dependent kinases CDK4 and CDK6. Moreover, we show that cancer-derived mutants of cyclin D1 are much more stable than wild-type cyclin D1 under anchorage-independent conditions and possess a greater antagonistic effect on FOXO-regulated anoikis and anchorage-independent growth of cancer cells. These data suggest that cyclin D1 may have a critical function in tumorigenesis and cancer metastasis by inhibiting the anoikis-promoting function of FOXO proteins.

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Cyclin D1 promotes anchorage-independent cell survival by inhibiting FOXO-mediated anoikis

Abstract

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