Cyclic AMP/GMP-dependent modulation of Ca2+ channels sets the polarity of nerve growth-cone turning

Makoto Nishiyama, Akemi Hoshino, Lily Tsai, John R Henley, Yoshio Goshima, Marc Tessier-Lavigne, Mu Ming Poo, Kyonsoo Hong

Research output: Contribution to journalArticle

312 Citations (Scopus)

Abstract

Signalling by intracellular second messengers such as cyclic nucleotides and Ca2+ is known to regulate attractive and repulsive guidance of axons by extracellular factors. However, the mechanism of interaction among these second messengers in determining the polarity of the guidance response is largely unknown. Here, we report that the ratio of cyclic AMP to cyclic GMP activities sets the polarity of netrin-1-induced axon guidance: high ratios favour attraction, whereas low ratios favour repulsion. Whole-cell recordings of Ca2+ currents at Xenopus spinal neuron growth cones indicate that cyclic nucleotide signalling directly modulates the activity of L-type Ca2+ channels (LCCs) in axonal growth cones. Furthermore, cGMP signalling activated by an arachidonate 12-lipoxygenase metabolite suppresses LCC activity triggered by netrin-1, and is required for growth-cone repulsion mediated by the DCC-UNC5 receptor complex. By linking cAMP and cGMP signalling and modulation of Ca2+ channel activity in growth cones, these findings delineate an early membrane-associated event responsible for signal transduction during bi-directional axon guidance.

Original languageEnglish (US)
Pages (from-to)990-995
Number of pages6
JournalNature
Volume423
Issue number6943
DOIs
StatePublished - Jun 26 2003
Externally publishedYes

Fingerprint

Growth Cones
Cyclic Nucleotides
Second Messenger Systems
Arachidonate 12-Lipoxygenase
Cyclic GMP
Patch-Clamp Techniques
Xenopus
Cyclic AMP
Signal Transduction
Neurons
Membranes
cyclic guanosine monophosphate-adenosine monophosphate
Axon Guidance
netrin-1

ASJC Scopus subject areas

  • General

Cite this

Nishiyama, M., Hoshino, A., Tsai, L., Henley, J. R., Goshima, Y., Tessier-Lavigne, M., ... Hong, K. (2003). Cyclic AMP/GMP-dependent modulation of Ca2+ channels sets the polarity of nerve growth-cone turning. Nature, 423(6943), 990-995. https://doi.org/10.1038/nature01751

Cyclic AMP/GMP-dependent modulation of Ca2+ channels sets the polarity of nerve growth-cone turning. / Nishiyama, Makoto; Hoshino, Akemi; Tsai, Lily; Henley, John R; Goshima, Yoshio; Tessier-Lavigne, Marc; Poo, Mu Ming; Hong, Kyonsoo.

In: Nature, Vol. 423, No. 6943, 26.06.2003, p. 990-995.

Research output: Contribution to journalArticle

Nishiyama, M, Hoshino, A, Tsai, L, Henley, JR, Goshima, Y, Tessier-Lavigne, M, Poo, MM & Hong, K 2003, 'Cyclic AMP/GMP-dependent modulation of Ca2+ channels sets the polarity of nerve growth-cone turning', Nature, vol. 423, no. 6943, pp. 990-995. https://doi.org/10.1038/nature01751
Nishiyama M, Hoshino A, Tsai L, Henley JR, Goshima Y, Tessier-Lavigne M et al. Cyclic AMP/GMP-dependent modulation of Ca2+ channels sets the polarity of nerve growth-cone turning. Nature. 2003 Jun 26;423(6943):990-995. https://doi.org/10.1038/nature01751
Nishiyama, Makoto ; Hoshino, Akemi ; Tsai, Lily ; Henley, John R ; Goshima, Yoshio ; Tessier-Lavigne, Marc ; Poo, Mu Ming ; Hong, Kyonsoo. / Cyclic AMP/GMP-dependent modulation of Ca2+ channels sets the polarity of nerve growth-cone turning. In: Nature. 2003 ; Vol. 423, No. 6943. pp. 990-995.
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