Cyclic ADP-ribose signaling in sea urchin gametes: Metabolism in spermatozoa

Eduardo N. Chini, Michael A. Thompson, Claudia C.S. Chini, Thomas P. Dousa

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

The molecular mechanism that initiates Ca2+ signaling in sea urchin egg fertilization has not yet been clarified. To determine whether sea urchin sperm may generate and possibly supply cyclic ADP-ribose (cADPR) as a Ca2+- releasing factor in the course of sea urchin egg fertilization, we determined cADPR content and the capacity for cADPR synthesis in sea urchin sperm. cADPR content was determined using the sea urchin egg homogenate Ca2+-release bioassay combined with high-performance liquid chromatography (HPLC). We found that sperm homogenates synthesized cADPR from β-NAD but did not synthesize cADPR when α-NAD was the substrate. The identity of cADPR generated by sperm homogenates was verified by HPLC analysis, use of specific Ca2+-release antagonists, and homologous desensitization of the sea urchin egg homogenate Ca2+-release bioassay. The ambient content of cADPR was ~0.3 nmol cADPR/g wet wt sea urchin sperm. Our results show that sperm can synthesize cADPR and that they contain cADPR levels comparable to other tissues.

Original languageEnglish (US)
Pages (from-to)C416-C420
JournalAmerican Journal of Physiology - Cell Physiology
Volume272
Issue number2 41-2
DOIs
StatePublished - Feb 1997

Keywords

  • adenosine diphosphoribose cyclase
  • calcium ion release
  • cyclic adenosine diphosphosphoribose
  • egg
  • fertilization
  • nicotinate adenine dinucleotide phosphate
  • ryanodine
  • sperm

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

Fingerprint Dive into the research topics of 'Cyclic ADP-ribose signaling in sea urchin gametes: Metabolism in spermatozoa'. Together they form a unique fingerprint.

  • Cite this