Cyclic ADP ribose induces Ca2+ release in CARDIAC muscle

Y.s. Prakash, Carlos Bernardo Mantilla, Y. S. Han, M. S. Kannan, T. F. Walseth, Gary C Sieck

Research output: Contribution to journalArticle

Abstract

Previous studies have reported that cyclic ADP-ribose (cADPR) induces Ca2+ release from the sarcoplasmic reticulum (SR) in cardiac myocytes. However, the involvement of ryanodine receptor (RyR) channels in the cADPR response remains controversial. In rat myocardium, cADPR-induced [Ca2+]i responses were assessed by: 1) measurement of [Ca2+]i in acutely dissociated myocytes using confocal microscopy; and 2) measurement of force responses in β-escin permeabilized ventricular strips. In dissociated myocytes, cADPR (0.1 to 10 μM) induced sustained, dose-dependent SR Ca2+ release, which was completely inhibited by 1 μM 8-amino cADPR, but only partially inhibited by 10 μM ryanodine. In ventricular strips, cADPR (10, 25 and 100 μM) induced sustained, dose-dependent force responses, which were completely inhibited by 50 μM 8-amino cADPR, but only partially inhibited by 10 μM ryanodine or 25 μM ruthenium red. With both ryanodine and ruthenium red, complete inhibition of RyR channels was confirmed by the lack of response to caffeine. These results indicate that in cardiac myocytes cADPR induces Ca2+ release through RyR channels. However, cADPR also appears to induce Ca2+ release through mechanisms independent of RyR channels.

Original languageEnglish (US)
JournalFASEB Journal
Volume12
Issue number5
StatePublished - Mar 20 1998
Externally publishedYes

Fingerprint

Cyclic ADP-Ribose
ribose
Muscle
Ryanodine Receptor Calcium Release Channel
calcium
Muscles
muscles
ryanodine receptors
Ryanodine
Ruthenium Red
Sarcoplasmic Reticulum
Cardiac Myocytes
sarcoplasmic reticulum
Muscle Cells
Escin
myocytes
Confocal microscopy
Caffeine
Confocal Microscopy
Rats

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Prakash, Y. S., Mantilla, C. B., Han, Y. S., Kannan, M. S., Walseth, T. F., & Sieck, G. C. (1998). Cyclic ADP ribose induces Ca2+ release in CARDIAC muscle. FASEB Journal, 12(5).

Cyclic ADP ribose induces Ca2+ release in CARDIAC muscle. / Prakash, Y.s.; Mantilla, Carlos Bernardo; Han, Y. S.; Kannan, M. S.; Walseth, T. F.; Sieck, Gary C.

In: FASEB Journal, Vol. 12, No. 5, 20.03.1998.

Research output: Contribution to journalArticle

Prakash, YS, Mantilla, CB, Han, YS, Kannan, MS, Walseth, TF & Sieck, GC 1998, 'Cyclic ADP ribose induces Ca2+ release in CARDIAC muscle', FASEB Journal, vol. 12, no. 5.
Prakash YS, Mantilla CB, Han YS, Kannan MS, Walseth TF, Sieck GC. Cyclic ADP ribose induces Ca2+ release in CARDIAC muscle. FASEB Journal. 1998 Mar 20;12(5).
Prakash, Y.s. ; Mantilla, Carlos Bernardo ; Han, Y. S. ; Kannan, M. S. ; Walseth, T. F. ; Sieck, Gary C. / Cyclic ADP ribose induces Ca2+ release in CARDIAC muscle. In: FASEB Journal. 1998 ; Vol. 12, No. 5.
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