CXCR4+ and FLK-1+ identify circulating cells associated with improved cardiac function in patients following myocardial infarction

Rahul Suresh, Anca Chiriac, Kashish Goel, Hector R Vilarraga, Francisco Lopez-Jimenez, Randal J. Thomas, Andre Terzic, Timothy J Nelson, Carmen M Terzic

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The biomarkers CXCR4/FLK-1 select cardiac progenitors from a stem cell pool in experimental models. However, the translational value of these cells in human ischemic heart disease is unknown. Here, flow-cytometry identified CD45-/CXCR4+/FLK-1+ cells in 30 individuals without ischemic heart disease and 33 first-time acute myocardial infarction (AMI) patients. AMI patients had higher CD45-/CXCR4 +/FLK-1+ cell-load at 48-h and 3- and 6-months post-AMI (p = 0.003,0.04,0.04, respectively) than controls. Cardiovascular risk factors and left ventricular (LV) ejection fraction were not associated with cell-load. 2D-speckle-tracking strain echocardiography assessment of LV systolic function showed improvement in longitudinal strain and dyssynchrony during follow-up associated with longitudinal increases in and higher 48-h post-AMI CD45 -/CXCR4+/FLK-1+ cell-load (r = -0.525, p = 0.025; r = -0.457, p = 0.029, respectively). In conclusion, CD45 -/CXCR4+/FLK-1+ cells are present in adult human circulation, increased in AMI and associated with improved LV systolic function. Thus, CD45-/CXCR4+/FLK-1+ cells may provide a diagnostic tool to follow cardiac regenerative capacity and potentially serve as a prognostic marker in AMI.

Original languageEnglish (US)
Pages (from-to)787-797
Number of pages11
JournalJournal of Cardiovascular Translational Research
Volume6
Issue number5
DOIs
StatePublished - Oct 2013

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Myocardial Infarction
Left Ventricular Function
Myocardial Ischemia
Stroke Volume
Echocardiography
Flow Cytometry
Theoretical Models
Stem Cells
Biomarkers

Keywords

  • Cardiac progenitor cells
  • CXCR4
  • FLK-1
  • Longitudinal strain
  • Myocardial infarction

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Genetics
  • Genetics(clinical)
  • Molecular Medicine
  • Pharmaceutical Science

Cite this

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title = "CXCR4+ and FLK-1+ identify circulating cells associated with improved cardiac function in patients following myocardial infarction",
abstract = "The biomarkers CXCR4/FLK-1 select cardiac progenitors from a stem cell pool in experimental models. However, the translational value of these cells in human ischemic heart disease is unknown. Here, flow-cytometry identified CD45-/CXCR4+/FLK-1+ cells in 30 individuals without ischemic heart disease and 33 first-time acute myocardial infarction (AMI) patients. AMI patients had higher CD45-/CXCR4 +/FLK-1+ cell-load at 48-h and 3- and 6-months post-AMI (p = 0.003,0.04,0.04, respectively) than controls. Cardiovascular risk factors and left ventricular (LV) ejection fraction were not associated with cell-load. 2D-speckle-tracking strain echocardiography assessment of LV systolic function showed improvement in longitudinal strain and dyssynchrony during follow-up associated with longitudinal increases in and higher 48-h post-AMI CD45 -/CXCR4+/FLK-1+ cell-load (r = -0.525, p = 0.025; r = -0.457, p = 0.029, respectively). In conclusion, CD45 -/CXCR4+/FLK-1+ cells are present in adult human circulation, increased in AMI and associated with improved LV systolic function. Thus, CD45-/CXCR4+/FLK-1+ cells may provide a diagnostic tool to follow cardiac regenerative capacity and potentially serve as a prognostic marker in AMI.",
keywords = "Cardiac progenitor cells, CXCR4, FLK-1, Longitudinal strain, Myocardial infarction",
author = "Rahul Suresh and Anca Chiriac and Kashish Goel and Vilarraga, {Hector R} and Francisco Lopez-Jimenez and Thomas, {Randal J.} and Andre Terzic and Nelson, {Timothy J} and Terzic, {Carmen M}",
year = "2013",
month = "10",
doi = "10.1007/s12265-013-9502-z",
language = "English (US)",
volume = "6",
pages = "787--797",
journal = "Journal of Cardiovascular Translational Research",
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T1 - CXCR4+ and FLK-1+ identify circulating cells associated with improved cardiac function in patients following myocardial infarction

AU - Suresh, Rahul

AU - Chiriac, Anca

AU - Goel, Kashish

AU - Vilarraga, Hector R

AU - Lopez-Jimenez, Francisco

AU - Thomas, Randal J.

AU - Terzic, Andre

AU - Nelson, Timothy J

AU - Terzic, Carmen M

PY - 2013/10

Y1 - 2013/10

N2 - The biomarkers CXCR4/FLK-1 select cardiac progenitors from a stem cell pool in experimental models. However, the translational value of these cells in human ischemic heart disease is unknown. Here, flow-cytometry identified CD45-/CXCR4+/FLK-1+ cells in 30 individuals without ischemic heart disease and 33 first-time acute myocardial infarction (AMI) patients. AMI patients had higher CD45-/CXCR4 +/FLK-1+ cell-load at 48-h and 3- and 6-months post-AMI (p = 0.003,0.04,0.04, respectively) than controls. Cardiovascular risk factors and left ventricular (LV) ejection fraction were not associated with cell-load. 2D-speckle-tracking strain echocardiography assessment of LV systolic function showed improvement in longitudinal strain and dyssynchrony during follow-up associated with longitudinal increases in and higher 48-h post-AMI CD45 -/CXCR4+/FLK-1+ cell-load (r = -0.525, p = 0.025; r = -0.457, p = 0.029, respectively). In conclusion, CD45 -/CXCR4+/FLK-1+ cells are present in adult human circulation, increased in AMI and associated with improved LV systolic function. Thus, CD45-/CXCR4+/FLK-1+ cells may provide a diagnostic tool to follow cardiac regenerative capacity and potentially serve as a prognostic marker in AMI.

AB - The biomarkers CXCR4/FLK-1 select cardiac progenitors from a stem cell pool in experimental models. However, the translational value of these cells in human ischemic heart disease is unknown. Here, flow-cytometry identified CD45-/CXCR4+/FLK-1+ cells in 30 individuals without ischemic heart disease and 33 first-time acute myocardial infarction (AMI) patients. AMI patients had higher CD45-/CXCR4 +/FLK-1+ cell-load at 48-h and 3- and 6-months post-AMI (p = 0.003,0.04,0.04, respectively) than controls. Cardiovascular risk factors and left ventricular (LV) ejection fraction were not associated with cell-load. 2D-speckle-tracking strain echocardiography assessment of LV systolic function showed improvement in longitudinal strain and dyssynchrony during follow-up associated with longitudinal increases in and higher 48-h post-AMI CD45 -/CXCR4+/FLK-1+ cell-load (r = -0.525, p = 0.025; r = -0.457, p = 0.029, respectively). In conclusion, CD45 -/CXCR4+/FLK-1+ cells are present in adult human circulation, increased in AMI and associated with improved LV systolic function. Thus, CD45-/CXCR4+/FLK-1+ cells may provide a diagnostic tool to follow cardiac regenerative capacity and potentially serve as a prognostic marker in AMI.

KW - Cardiac progenitor cells

KW - CXCR4

KW - FLK-1

KW - Longitudinal strain

KW - Myocardial infarction

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