CXCR4 is required by a nonprimate lentivirus: Heterologous expression of feline immunodeficiency virus in human, rodent, and feline cells

Eric M. Poeschla, David J. Looney

Research output: Contribution to journalArticle

86 Citations (Scopus)

Abstract

A heterologous feline immunodeficiency virus (FIV) expression system permitted high-level expression of FIV proteins and efficient production of infectious FIV in human cells. These results identify the FIV U3 element as the sole restriction to the productive phase of replication in nonfeline cells. Heterologous FIV expression in a variety of human cell lines resulted in profuse syncytial lysis that was FIV env specific, CD4 independent, and restricted to cells that express CXCR4, the coreceptor for T-cell-line- adapted strains of human immunodeficiency virus. Stable expression of human CXCR4 in CXCR4-negative human and rodent cell lines resulted in extensive FIV Env-mediated, CXCR4-dependent cell fusion and infection. In feline cells, stable overexpression of human CXCR4 resulted in increased FIV infectivity and marked syncytium formation during FIV replication or after infection with FIV Env-expressing vectors. The use of CXCR4 is a fundamental feature of lentivirus biology independent of CD4 and a shared cellular link to infection and cytopathicity for distantly related lentiviruses that cause AIDS. Their conserved use implicates chemokine receptors as primordial lentivirus receptors.

Original languageEnglish (US)
Pages (from-to)6858-6866
Number of pages9
JournalJournal of Virology
Volume72
Issue number8
StatePublished - Aug 1998
Externally publishedYes

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Feline Immunodeficiency Virus
Lentivirus
Feline immunodeficiency virus
Felidae
Rodentia
rodents
cats
cells
Cell Line
Infection
cell lines
infection
cell fusion
human cell lines
Cell Fusion
Chemokine Receptors
Human immunodeficiency virus
giant cells
Giant Cells
Virus Replication

ASJC Scopus subject areas

  • Immunology

Cite this

CXCR4 is required by a nonprimate lentivirus : Heterologous expression of feline immunodeficiency virus in human, rodent, and feline cells. / Poeschla, Eric M.; Looney, David J.

In: Journal of Virology, Vol. 72, No. 8, 08.1998, p. 6858-6866.

Research output: Contribution to journalArticle

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