CXCL12-mediated monocyte transmigration into brain perivascular space leads to neuroinflammation and memory deficit in neuropathic pain

Chun Lin Mai, Zhi Tan, Ya Nan Xu, Jing Jun Zhou, Zhen Hua Huang, Dong Wang, Hui Zhang, Wen Shan Gui, Jun Zhang, Zhen Jia Lin, Ying Tong Meng, Xiao Wei, Ying Tao Jie, Peter M. Grace, Long Jun Wu, Li Jun Zhou, Xian Guo Liu

Research output: Contribution to journalArticlepeer-review

Abstract

Emerging clinical and experimental evidence demonstrates that neuroinflammation plays an important role in cognitive impairment associated with neuropathic pain. However, how peripheral nerve challenge induces remote inflammation in the brain remains largely unknown. Methods: The circulating leukocytes and plasma C-X-C motif chemokine 12 (CXCL12) and brain perivascular macrophages (PVMs) were analyzed by flow cytometry, Western blotting, ELISA, and immunostaining in spared nerve injury (SNI) mice. The memory function was evaluated with a novel object recognition test (NORT) in mice and with Montreal Cognitive Assessment (MoCA) in chronic pain patients. Results: The classical monocytes and CXCL12 in the blood, PVMs in the perivascular space, and gliosis in the brain, particularly in the hippocampus, were persistently increased following SNI in mice. Using the transgenic CCR2RFP/+ and CX3CR1GFP/+ mice, we discovered that at least some of the PVMs were recruited from circulating monocytes. The SNI-induced increase in hippocampal PVMs, gliosis, and memory decline were substantially prevented by either depleting circulating monocytes via intravenous injection of clodronate liposomes or blockade of CXCL12-CXCR4 signaling. On the contrary, intravenous injection of CXCL12 at a pathological concentration in naïve mice mimicked SNI effects. Significantly, we found that circulating monocytes and plasma CXCL12 were elevated in chronic pain patients, and both of them were closely correlated with memory decline. Conclusion: CXCL12-mediated monocyte recruitment into the perivascular space is critical for neuroinflammation and the resultant cognitive impairment in neuropathic pain.

Original languageEnglish (US)
Pages (from-to)1059-1078
Number of pages20
JournalTheranostics
Volume11
Issue number3
DOIs
StatePublished - Jan 1 2021

Keywords

  • CXCL12
  • Chronic pain
  • Hippocampus perivascular macrophage
  • Memory deficit
  • Monocyte transmigration
  • Vneuroinflammation

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Pharmacology, Toxicology and Pharmaceutics (miscellaneous)

Fingerprint Dive into the research topics of 'CXCL12-mediated monocyte transmigration into brain perivascular space leads to neuroinflammation and memory deficit in neuropathic pain'. Together they form a unique fingerprint.

Cite this