Cultured human fibroblasts secrete insulin-like growth factor IA prohormone

Cheryl A Conover, B. K. Baker, R. L. Hintz

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Nucleotide sequencing of the two known cDNAs encoding human insulin-like growth factor I (IGF-I) predicts the existence of two different prohormone forms of IGF-I. The E peptide regions extend the carboxy-terminus of IGF-I by either an additional 35 (IGF-IA) or 77 (IGF-IB) amino acids. With a specific and sensitive RIA employing and antiserum directed against a synthetic peptide that is unique to the E peptide region of IGF-IA prohormone (EIA), we have identified EIA-immunoreactive material in the conditioned medium of fetal and postnatal human fibroblasts in culture. Incubation of postnatal human fibroblasts with GH increased specific immunoreactive EIA secretion 2- to 3-fold. There was no immunologically detectable 7.5K IGF-I or IGF-II peptide in acid-chromatographed human fibroblast-conditioned medium under either basal or GH-stimulated conditions. Acid chromatography of human fibroblast-conditioned medium on Sephadex G-75 revealed a single elution peak of EIA immunoreactivity corresponding to a mol wt of 9-17K. With neutral chromatography, EIA immunoreactivity eluted at 25-38K mol wt. These data suggest that the E peptide region of IGF-IA is translated and released as part of the prohormone form in cultured human fibroblasts, and that the levels of this prohormone are regulated by GH.

Original languageEnglish (US)
Pages (from-to)25-30
Number of pages6
JournalJournal of Clinical Endocrinology and Metabolism
Volume69
Issue number1
StatePublished - 1989
Externally publishedYes

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Somatomedins
Fibroblasts
Insulin-Like Growth Factor I
Conditioned Culture Medium
Chromatography
Peptides
Acids
Insulin-Like Growth Factor II
Tocopherols
Cell culture
Immune Sera
Nucleotides
Complementary DNA
Amino Acids
peptide E (adrenal medulla)

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Cultured human fibroblasts secrete insulin-like growth factor IA prohormone. / Conover, Cheryl A; Baker, B. K.; Hintz, R. L.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 69, No. 1, 1989, p. 25-30.

Research output: Contribution to journalArticle

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