Cryogenic micro-CT imaging of tissues

Steven M. Jorgensen, Russell T. Turner, Erik L. Ritman

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In order to facilitate the full use of histological methods following micro-CT imaging a cryogenic micro-CT scanning method is presented. The method involves rapid removal of the rodent organ (or biopsy from a large animal) and cooling it down rapidly. Next, the specimen is placed inside a specially-designed vessel and maintained at liquid N2 temperature. This vessel consists of a double-walled copper container with a vacuum space between the walls which is evacuated. Beryllium 'windows' in the vessel walls minimize x-ray attenuation of the low energy x-ray photons. The specimen is mounted on a stainless steel rod so that the specimen can be rotated in the small angular steps required in the CT scanning process.

Original languageEnglish (US)
JournalAnnals of Biomedical Engineering
Volume28
Issue numberSUPPL. 1
StatePublished - 2000

Fingerprint

Cryogenics
Tissue
Scanning
Imaging techniques
X rays
Biopsy
Beryllium
Containers
Animals
Stainless steel
Photons
Vacuum
Cooling
Copper
Liquids
Temperature
Rodentia

ASJC Scopus subject areas

  • Biomedical Engineering

Cite this

Jorgensen, S. M., Turner, R. T., & Ritman, E. L. (2000). Cryogenic micro-CT imaging of tissues. Annals of Biomedical Engineering, 28(SUPPL. 1).

Cryogenic micro-CT imaging of tissues. / Jorgensen, Steven M.; Turner, Russell T.; Ritman, Erik L.

In: Annals of Biomedical Engineering, Vol. 28, No. SUPPL. 1, 2000.

Research output: Contribution to journalArticle

Jorgensen, SM, Turner, RT & Ritman, EL 2000, 'Cryogenic micro-CT imaging of tissues', Annals of Biomedical Engineering, vol. 28, no. SUPPL. 1.
Jorgensen SM, Turner RT, Ritman EL. Cryogenic micro-CT imaging of tissues. Annals of Biomedical Engineering. 2000;28(SUPPL. 1).
Jorgensen, Steven M. ; Turner, Russell T. ; Ritman, Erik L. / Cryogenic micro-CT imaging of tissues. In: Annals of Biomedical Engineering. 2000 ; Vol. 28, No. SUPPL. 1.
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