Cryoelectron microscopy of protein IX-modified adenoviruses suggests a new position for the C terminus of protein IX

Michael P. Marsh, Samuel K. Campos, Matthew L. Baker, Christopher Y. Chen, Wah Chiu, Michael A Barry

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Recombinant human adenovirus is a useful gene delivery vector for clinical gene therapy. Minor capsid protein IX of adenovirus has been of recent interest since multiple studies have shown that modifications can be made to its C terminus to alter viral tropism or add molecular tags and/or reporter proteins. We examined the structure of an engineered adenovirus displaying the enhanced green fluorescent protein (EGFP) fused to the C terminus of protein IX. Cryoelectron microscopy and reconstruction localized the C-terminal EGFP fusion between the H2 hexon and the H4 hexon, positioned between adjacent facets, directly above the density previously assigned as protein IIIa. The original assignment of IIIa was based largely on indirect evidence, and the data presented herein support the reassignment of the IIIa density as protein IX.

Original languageEnglish (US)
Pages (from-to)11881-11886
Number of pages6
JournalJournal of Virology
Volume80
Issue number23
DOIs
StatePublished - Dec 2006

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Cryoelectron Microscopy
Adenoviridae
Protein C
Viral Tropism
green fluorescent protein
Human Adenoviruses
Proteins
manmade structures
proteins
Capsid Proteins
Genetic Therapy
tropisms
gene therapy
coat proteins
gene transfer
Genes
cryo-electron microscopy
enhanced green fluorescent protein

ASJC Scopus subject areas

  • Immunology

Cite this

Cryoelectron microscopy of protein IX-modified adenoviruses suggests a new position for the C terminus of protein IX. / Marsh, Michael P.; Campos, Samuel K.; Baker, Matthew L.; Chen, Christopher Y.; Chiu, Wah; Barry, Michael A.

In: Journal of Virology, Vol. 80, No. 23, 12.2006, p. 11881-11886.

Research output: Contribution to journalArticle

Marsh, Michael P. ; Campos, Samuel K. ; Baker, Matthew L. ; Chen, Christopher Y. ; Chiu, Wah ; Barry, Michael A. / Cryoelectron microscopy of protein IX-modified adenoviruses suggests a new position for the C terminus of protein IX. In: Journal of Virology. 2006 ; Vol. 80, No. 23. pp. 11881-11886.
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