Cross-species analysis of nicotine-induced proteomic alterations in pancreatic cells

Joao A. Paulo, Raul Urrutia, Vivek Kadiyala, Peter Banks, Darwin L. Conwell, Hanno Steen

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Toxic compounds in tobacco, such as nicotine, may adversely affect pancreatic function. We aim to determine nicotine-induced protein alterations in pancreatic cells, thereby revealing links between nicotine exposure and pancreatic disease. We compared the proteomic alterations induced by nicotine treatment in cultured pancreatic cells (mouse, rat, and human stellate cells and human duct cells) using MS-based techniques, specifically SDS-PAGE (gel) coupled with LC-MS/MS and spectral counting. We identified thousands of proteins in pancreatic cells, hundreds of which were identified exclusively or in higher abundance in either nicotine-treated or untreated cells. Interspecies comparisons of stellate cell proteins revealed several differentially abundant proteins (in nicotine treated versus untreated cells) common among the three species. Proteins appearing in all nicotine-treated stellate cells include amyloid beta (A4), procollagen type VI alpha 1, integral membrane protein 2B, and toll-interacting protein. Proteins that were differentially expressed upon nicotine treatment across cell lines were enriched in certain pathways, including nicotinic acetylcholine receptor, cytokine, and integrin signaling. At this analytical depth, we conclude that similar pathways are affected by nicotine, but alterations at the protein level among stellate cells of different species vary. Further interrogation of such pathways will lead to insights into the potential effect of nicotine on pancreatic cells at the biomolecular level and the extension of this concept to the effect of nicotine on pancreatic disease.

Original languageEnglish (US)
Pages (from-to)1499-1512
Number of pages14
JournalProteomics
Volume13
Issue number9
DOIs
StatePublished - May 2013

Fingerprint

Nicotine
Proteomics
Proteins
Pancreatic Diseases
Collagen Type VI
Tobacco
Poisons
Nicotinic Receptors
Amyloid
Integrins
Ducts
Rats
Polyacrylamide Gel Electrophoresis
Cultured Cells
Membrane Proteins
Gels
Cells
Cytokines
Cell Line

Keywords

  • Biomarker
  • Cell biology
  • Chronic pancreatitis
  • Cross-species comparison
  • Pancreas
  • Pancreatic cancer

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry

Cite this

Paulo, J. A., Urrutia, R., Kadiyala, V., Banks, P., Conwell, D. L., & Steen, H. (2013). Cross-species analysis of nicotine-induced proteomic alterations in pancreatic cells. Proteomics, 13(9), 1499-1512. https://doi.org/10.1002/pmic.201200492

Cross-species analysis of nicotine-induced proteomic alterations in pancreatic cells. / Paulo, Joao A.; Urrutia, Raul; Kadiyala, Vivek; Banks, Peter; Conwell, Darwin L.; Steen, Hanno.

In: Proteomics, Vol. 13, No. 9, 05.2013, p. 1499-1512.

Research output: Contribution to journalArticle

Paulo, JA, Urrutia, R, Kadiyala, V, Banks, P, Conwell, DL & Steen, H 2013, 'Cross-species analysis of nicotine-induced proteomic alterations in pancreatic cells', Proteomics, vol. 13, no. 9, pp. 1499-1512. https://doi.org/10.1002/pmic.201200492
Paulo JA, Urrutia R, Kadiyala V, Banks P, Conwell DL, Steen H. Cross-species analysis of nicotine-induced proteomic alterations in pancreatic cells. Proteomics. 2013 May;13(9):1499-1512. https://doi.org/10.1002/pmic.201200492
Paulo, Joao A. ; Urrutia, Raul ; Kadiyala, Vivek ; Banks, Peter ; Conwell, Darwin L. ; Steen, Hanno. / Cross-species analysis of nicotine-induced proteomic alterations in pancreatic cells. In: Proteomics. 2013 ; Vol. 13, No. 9. pp. 1499-1512.
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