Cross-reactions of anti-I-E[k] monoclonal antibodies with subsets of I-A[b] molecules

W. P. Lafuse, M. Pierres, C. S. David

Research output: Contribution to journalArticle

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Abstract

Monoclonal antibodies H9-15.4 and H39-459 were derived from an A.TH anti-A.TL immunization. Antibodies H9-15.4 and H39-459 were found to be directed against the I-E[k] molecule with positive reactions against B10.A (A[k]E[k]) and B10.S(9R) (A[s]E[k]) but not B10.A(4R) (A[k]E[b]). The monoclonal antibodies were also found to react with the b and q haplotypes, which do not express an I-E molecule. Sequential precipitation analysis showed that in the b haplotype, H9-15.4 and H39-459 react with the I-A[b] molecule. These results showed that H9-15.4 and H39-459 recognize determinants shared by I-E[k] and I-A[b] molecules, suggesting that I-A and I-E molecules may have a common evolutionary origin, possibly through gene duplication. Sequential immunoprecipitation analysis of I-A[b] molecules precipitated by H9-15.4 and H39-459 also suggested that these monoclonal antibodies recognize subsets of I-A[b] molecules. Pretreatment with the 17-227 monoclonal anti-I-A[b] antibody (Ia.15) had no effect on I-A[b] molecules precipitated by H9-15.4 and H39-459. Also, pretreatment with H9-15.4 and H39-459 had no effect on I-A[b] molecules immunoprecipitated by 17-227. Also H9-15.4 and H39-459 only partially cleared Ia molecules immunoprecipitated by each other. These results suggest that 17-227, H9-15.4, and H39-459 detect a minimum of four subsets of I-A[b] molecules. To account for these observations, it is proposed that during evolution of the mouse H-2 complex, in addition to gene duplication of ancestral gene(s) to yield the genes encoding E(β) E(α)A(α)A(β) polypeptide chains, further gene duplication occurred, forming multiple copies of genes encoding each Ia polypeptide chain.

Original languageEnglish (US)
Pages (from-to)49-55
Number of pages7
JournalTransplantation
Volume35
Issue number1
StatePublished - 1983

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Gene Duplication
Cross Reactions
Monoclonal Antibodies
Haplotypes
Genes
Peptides
Antibodies
Immunoprecipitation
Immunization

ASJC Scopus subject areas

  • Immunology
  • Transplantation

Cite this

Lafuse, W. P., Pierres, M., & David, C. S. (1983). Cross-reactions of anti-I-E[k] monoclonal antibodies with subsets of I-A[b] molecules. Transplantation, 35(1), 49-55.

Cross-reactions of anti-I-E[k] monoclonal antibodies with subsets of I-A[b] molecules. / Lafuse, W. P.; Pierres, M.; David, C. S.

In: Transplantation, Vol. 35, No. 1, 1983, p. 49-55.

Research output: Contribution to journalArticle

Lafuse, WP, Pierres, M & David, CS 1983, 'Cross-reactions of anti-I-E[k] monoclonal antibodies with subsets of I-A[b] molecules', Transplantation, vol. 35, no. 1, pp. 49-55.
Lafuse, W. P. ; Pierres, M. ; David, C. S. / Cross-reactions of anti-I-E[k] monoclonal antibodies with subsets of I-A[b] molecules. In: Transplantation. 1983 ; Vol. 35, No. 1. pp. 49-55.
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abstract = "Monoclonal antibodies H9-15.4 and H39-459 were derived from an A.TH anti-A.TL immunization. Antibodies H9-15.4 and H39-459 were found to be directed against the I-E[k] molecule with positive reactions against B10.A (A[k]E[k]) and B10.S(9R) (A[s]E[k]) but not B10.A(4R) (A[k]E[b]). The monoclonal antibodies were also found to react with the b and q haplotypes, which do not express an I-E molecule. Sequential precipitation analysis showed that in the b haplotype, H9-15.4 and H39-459 react with the I-A[b] molecule. These results showed that H9-15.4 and H39-459 recognize determinants shared by I-E[k] and I-A[b] molecules, suggesting that I-A and I-E molecules may have a common evolutionary origin, possibly through gene duplication. Sequential immunoprecipitation analysis of I-A[b] molecules precipitated by H9-15.4 and H39-459 also suggested that these monoclonal antibodies recognize subsets of I-A[b] molecules. Pretreatment with the 17-227 monoclonal anti-I-A[b] antibody (Ia.15) had no effect on I-A[b] molecules precipitated by H9-15.4 and H39-459. Also, pretreatment with H9-15.4 and H39-459 had no effect on I-A[b] molecules immunoprecipitated by 17-227. Also H9-15.4 and H39-459 only partially cleared Ia molecules immunoprecipitated by each other. These results suggest that 17-227, H9-15.4, and H39-459 detect a minimum of four subsets of I-A[b] molecules. To account for these observations, it is proposed that during evolution of the mouse H-2 complex, in addition to gene duplication of ancestral gene(s) to yield the genes encoding E(β) E(α)A(α)A(β) polypeptide chains, further gene duplication occurred, forming multiple copies of genes encoding each Ia polypeptide chain.",
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N2 - Monoclonal antibodies H9-15.4 and H39-459 were derived from an A.TH anti-A.TL immunization. Antibodies H9-15.4 and H39-459 were found to be directed against the I-E[k] molecule with positive reactions against B10.A (A[k]E[k]) and B10.S(9R) (A[s]E[k]) but not B10.A(4R) (A[k]E[b]). The monoclonal antibodies were also found to react with the b and q haplotypes, which do not express an I-E molecule. Sequential precipitation analysis showed that in the b haplotype, H9-15.4 and H39-459 react with the I-A[b] molecule. These results showed that H9-15.4 and H39-459 recognize determinants shared by I-E[k] and I-A[b] molecules, suggesting that I-A and I-E molecules may have a common evolutionary origin, possibly through gene duplication. Sequential immunoprecipitation analysis of I-A[b] molecules precipitated by H9-15.4 and H39-459 also suggested that these monoclonal antibodies recognize subsets of I-A[b] molecules. Pretreatment with the 17-227 monoclonal anti-I-A[b] antibody (Ia.15) had no effect on I-A[b] molecules precipitated by H9-15.4 and H39-459. Also, pretreatment with H9-15.4 and H39-459 had no effect on I-A[b] molecules immunoprecipitated by 17-227. Also H9-15.4 and H39-459 only partially cleared Ia molecules immunoprecipitated by each other. These results suggest that 17-227, H9-15.4, and H39-459 detect a minimum of four subsets of I-A[b] molecules. To account for these observations, it is proposed that during evolution of the mouse H-2 complex, in addition to gene duplication of ancestral gene(s) to yield the genes encoding E(β) E(α)A(α)A(β) polypeptide chains, further gene duplication occurred, forming multiple copies of genes encoding each Ia polypeptide chain.

AB - Monoclonal antibodies H9-15.4 and H39-459 were derived from an A.TH anti-A.TL immunization. Antibodies H9-15.4 and H39-459 were found to be directed against the I-E[k] molecule with positive reactions against B10.A (A[k]E[k]) and B10.S(9R) (A[s]E[k]) but not B10.A(4R) (A[k]E[b]). The monoclonal antibodies were also found to react with the b and q haplotypes, which do not express an I-E molecule. Sequential precipitation analysis showed that in the b haplotype, H9-15.4 and H39-459 react with the I-A[b] molecule. These results showed that H9-15.4 and H39-459 recognize determinants shared by I-E[k] and I-A[b] molecules, suggesting that I-A and I-E molecules may have a common evolutionary origin, possibly through gene duplication. Sequential immunoprecipitation analysis of I-A[b] molecules precipitated by H9-15.4 and H39-459 also suggested that these monoclonal antibodies recognize subsets of I-A[b] molecules. Pretreatment with the 17-227 monoclonal anti-I-A[b] antibody (Ia.15) had no effect on I-A[b] molecules precipitated by H9-15.4 and H39-459. Also, pretreatment with H9-15.4 and H39-459 had no effect on I-A[b] molecules immunoprecipitated by 17-227. Also H9-15.4 and H39-459 only partially cleared Ia molecules immunoprecipitated by each other. These results suggest that 17-227, H9-15.4, and H39-459 detect a minimum of four subsets of I-A[b] molecules. To account for these observations, it is proposed that during evolution of the mouse H-2 complex, in addition to gene duplication of ancestral gene(s) to yield the genes encoding E(β) E(α)A(α)A(β) polypeptide chains, further gene duplication occurred, forming multiple copies of genes encoding each Ia polypeptide chain.

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