Cross-linking the B7 family molecule B7-DC directly activates immune functions of dendritic cells

Loc T. Nguyen, Suresh Radhakrishnan, Bogoljub Ciric, Koji Tamada, Tahiro Shin, Drew M. Pardoll, Lieping Chen, Moses Rodriguez, Larry R. Pease

Research output: Contribution to journalArticle

86 Scopus citations

Abstract

B7-DC molecules are known to function as ligands on antigen-presenting cells (APCs), enhancing T cell activation. In this study, cross-linking B7-DC with the monoclonal antibody sHIgM12 directly potentiates dendritic cell (DC) function by enhancing DC presentation of major histocompatibility complex-peptide complexes, promoting DC survival; and increasing secretion of interleukin (IL)-12p70, a key T helper cell type 1 promoting cytokine. Furthermore, ex vivo treatment of DCs or systemic treatment of mice with sHIgM12 increases the number of transplanted DCs that reach draining lymph nodes and increases the ability of lymph node APCs to activate naive T cells. Systemic administration of the antibody has an equivalent effect on DCs transferred at a distant site. These findings implicate B7-DC expressed on DCs in bidirectional communication. In addition to the established costimulatory and inhibitory functions associated with B7-DC, this molecule can also function as a conduit for extracellular signals to DCs modifying DC functions.

Original languageEnglish (US)
Pages (from-to)1393-1398
Number of pages6
JournalJournal of Experimental Medicine
Volume196
Issue number10
DOIs
StatePublished - Nov 18 2002

Keywords

  • B7 superfamily
  • B7-DC
  • Costimulation
  • Dendritic cells
  • IL-12

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Nguyen, L. T., Radhakrishnan, S., Ciric, B., Tamada, K., Shin, T., Pardoll, D. M., Chen, L., Rodriguez, M., & Pease, L. R. (2002). Cross-linking the B7 family molecule B7-DC directly activates immune functions of dendritic cells. Journal of Experimental Medicine, 196(10), 1393-1398. https://doi.org/10.1084/jem.20021466