Cross-bridge kinetics in respiratory muscles

Gary C Sieck, Y.s. Prakash

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

In respiratory muscles, force generation and shortening depend on the cyclical interaction of actin and myosin (cross-bridge cycling). During cross-bridge cycling, adenosine triphosphate (ATP) is hydrolysed. The globular head region of the myosin heavy chain (MyHC) possesses both the binding site to actin and the site for ATP hydrolysis. Therefore, the MyHC is both a structural and enzymatic protein. Different isoforms of MyHC are expressed in skeletal muscle fibres, and these MyHC isoforms provide mechanical and metabolic diversity. In the present study, the relationships between MyHC isoform expression in single rat diaphragm muscle fibres and their mechanical and energetic properties were evaluated. The expression of MyHC isoforms in single diaphragm muscle fibres was identified using electrophoretic and immunohistochemical techniques. Cross-bridge cycling kinetics in diaphragm muscle fibres clearly depend on MyHC isoform expression, and these differences are interpreted in the context of Huxley's two state cross-bridge model. It is concluded that the unique mechanical and energetic properties of myosin heavy chain isoforms are designed to accomplish different motor behaviours of the diaphragm muscle, and that, as a result of these unique properties, a selective recruitment of diaphragm muscle fibres is essential to avoid fatigue.

Original languageEnglish (US)
Pages (from-to)2147-2158
Number of pages12
JournalEuropean Respiratory Journal
Volume10
Issue number9
DOIs
StatePublished - Sep 1997

Fingerprint

Respiratory Muscles
Myosin Heavy Chains
Diaphragm
Protein Isoforms
Muscles
Actins
Adenosine Triphosphate
Skeletal Muscle Myosins
Skeletal Muscle Fibers
Myosins
Fatigue
Hydrolysis
Binding Sites

Keywords

  • Diaphragm muscle
  • Fatigue
  • Fibre type
  • Force
  • Myosin heavy chain
  • Shortening velocity

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Cross-bridge kinetics in respiratory muscles. / Sieck, Gary C; Prakash, Y.s.

In: European Respiratory Journal, Vol. 10, No. 9, 09.1997, p. 2147-2158.

Research output: Contribution to journalArticle

Sieck, Gary C ; Prakash, Y.s. / Cross-bridge kinetics in respiratory muscles. In: European Respiratory Journal. 1997 ; Vol. 10, No. 9. pp. 2147-2158.
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