Abstract
This paper describes a novel approach of forming micropatterned co-cultures using a combination of oxygen plasma ashing and electrochemical removal of poly(ethylene glycol) (PEG) silane from indium tin oxide (ITO) substrates. After assembling a layer of PEG silane on ITO, photolithography and oxygen plasma cleaning were used to form cell-adhesive domains within the non-fouling PEG silane layer. After patterning hepatocytes in these domains, the surrounding PEG silane layer was desorbed by applying negative voltage (-1.4 V vs. Ag/AgCl) to the underlying ITO substrate. This switched the fouling properties of the ITO substrate and allowed the adsorption of another cell type creating a micropatterned co-culture.
| Original language | English (US) |
|---|---|
| Pages | 257-259 |
| Number of pages | 3 |
| State | Published - 2008 |
| Event | 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States Duration: Oct 12 2008 → Oct 16 2008 |
Other
| Other | 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 |
|---|---|
| Country/Territory | United States |
| City | San Diego, CA |
| Period | 10/12/08 → 10/16/08 |
Keywords
- Micropatterned co-culture
- Photolithography
- Switchable biointerface
ASJC Scopus subject areas
- Chemical Engineering (miscellaneous)
- Bioengineering