CpG DNA induces a class II transactivator-independent increase in class II MHC by stabilizing class II MHC mRNA in B lymphocytes

John Kuchtey, Meghan Pennini, Rish K. Pai, Clifford V. Harding

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Microbial products, such as CpG DNA and LPS, enhance class II MHC (MHC-II) expression and Ag presentation by dendritic cells, but this effect does not occur with macrophages and is largely unexplored in B cells. Although MHC-II expression is influenced by transcriptional regulation, which is governed by class II transactivator (CIITA) in all cells, microbial products enhance MHC-II expression by dendritic cells in part by increasing MHC-II protein stability. In this study, we show that the CpG-induced increase in MHC-II expression by B lymphocytes is not due to protein stabilization or changes in CIITA expression or activity, but instead is due to increased stability of MHC-II mRNA. This CIITA-independent mechanism adds a new layer of complexity to regulation of MHC-II and may increase T cell help for B cell Ab responses to microbial or vaccine Ags.

Original languageEnglish (US)
Pages (from-to)2320-2325
Number of pages6
JournalJournal of Immunology
Volume171
Issue number5
DOIs
StatePublished - Sep 1 2003

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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