COX inhibition increases alternaria-induced pulmonary group 2 innate lymphoid cell responses and IL-33 release in mice

Weisong Zhou, Jian Zhang, Shinji Toki, Kasia Goleniewska, Allison E. Norlander, Dawn C. Newcomb, Pingsheng Wu, Kelli L. Boyd, Hirohito Kita, R. Stokes Peebles

Research output: Contribution to journalArticle

Abstract

The cyclooxygenase (COX) metabolic pathway regulates immune responses and inflammation. The effect of the COX pathway on innate pulmonary inflammation induced by protease-containing fungal allergens, such as Alternaria alternata, is not fully defined. In this study, we tested the hypothesis that COX inhibition augments Alternaria-induced pulmonary group 2 innate lymphoid cell (ILC2) responses and IL-33 release. Mice were treated with the COX inhibitors indomethacin, flurbiprofen, or vehicle and challenged intranasally with Alternaria extract for four consecutive days to induce innate lung inflammation. We found that indomethacin and flurbiprofen significantly increased the numbers of ILC2 and IL-5 and IL-13 expression by ILC2 in the lung. Indomethacin also increased ILC2 proliferation, the percentages of eosinophils, and mucus production in the lung. Both indomethacin and flurbiprofen augmented the release of IL-33 in bronchoalveolar lavage fluid after Alternaria challenge, suggesting that more IL-33 was available for ILC2 activation and that a COX product(s) inhibited IL-33 release. This is supported by the in vitro finding that the COX product PGE2 and the PGI2 analogs cicaprost decreased Alternaria extract induced IL-33 release by human bronchial epithelial cells. Although contrasting effects of PGD2, PGE2, and PGI2 on ILC2 responses have been previously reported, the overall effect of the COX pathway on ILC2 function is inhibitory in Alternaria-induced innate airway inflammation.

Original languageEnglish (US)
Pages (from-to)1157-1166
Number of pages10
JournalJournal of Immunology
Volume205
Issue number4
DOIs
StatePublished - Aug 15 2020

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Zhou, W., Zhang, J., Toki, S., Goleniewska, K., Norlander, A. E., Newcomb, D. C., Wu, P., Boyd, K. L., Kita, H., & Peebles, R. S. (2020). COX inhibition increases alternaria-induced pulmonary group 2 innate lymphoid cell responses and IL-33 release in mice. Journal of Immunology, 205(4), 1157-1166. https://doi.org/10.4049/jimmunol.1901544