TY - JOUR
T1 - Correlation of TGF-(si expression with medroxiprogesterone acetate responsiveness in mouse mammary adenocarcinomas
AU - Elizalde, Patricia V.
AU - Guerra, Fabiana K.
AU - Gravano, Martin
AU - Lanari, Claudia
AU - Lippman, Marc E.
AU - Charreau, Eduardo H.
AU - Lupu, Ruth
N1 - Funding Information:
The authors thank Dr. Francis G. Kern for providing the TGF-PI and TGF-a riprobes and for help and advice with the Northern hybridizations. Dr. Michael Johnson for advice with the hybridizations. and Dr. Robert Clarke for providing the cDNA TGF-a probe. P. V. Elizalde thanks the Vincent T. Lombardi Cancer Research Center, Georgetown University Medical Center, for providing guest worker status during the performance of this work. P. V. Elizalde was a recipient of an International Cancer Research Technology Transfer Project (ICRETT) award from the International Union Against Cancer. This work was in part supported by Fundacion Antorchas and Consejo Nacional de lnvestigaciones Cientificas y Tecnicas from Argentina.
PY - 1995
Y1 - 1995
N2 - We investigated the expression of transforming growth factors β1 and α (TGF-β1, TGF-α in hormone-responsive (MPA-R) and unresponsive (MPA-U) tumor lines obtained from medroxyprogesterone acetate (MPA)-induced mammary adenocarcinomas in BALBIc mice. The tumors were transplanted into MPA-treated and untreated mice. TGF-β1 gene expression was observed in the MPA-R lines growing in untreated animals, but not in MPA-treated mice. TGF-β1 mRNA was not detected in the MPA-U tumor lines growing in either MPA-treated or untreated animals. In MPA-R lines the levels of TGF-β1 expression were inversely correlated to growth rate. High-affinity TGF-β1 receptors were present in the MPA-R tumors. These results suggest that one of the mechanisms by which MP A exerts its proliferative effect on MPA-R tumor lines is inhibition of the expression of TGF-β1. Thus, the lack of expression of TGF-β1 in MPA-U tumors may be related to the acquisition of autonomous growth.
AB - We investigated the expression of transforming growth factors β1 and α (TGF-β1, TGF-α in hormone-responsive (MPA-R) and unresponsive (MPA-U) tumor lines obtained from medroxyprogesterone acetate (MPA)-induced mammary adenocarcinomas in BALBIc mice. The tumors were transplanted into MPA-treated and untreated mice. TGF-β1 gene expression was observed in the MPA-R lines growing in untreated animals, but not in MPA-treated mice. TGF-β1 mRNA was not detected in the MPA-U tumor lines growing in either MPA-treated or untreated animals. In MPA-R lines the levels of TGF-β1 expression were inversely correlated to growth rate. High-affinity TGF-β1 receptors were present in the MPA-R tumors. These results suggest that one of the mechanisms by which MP A exerts its proliferative effect on MPA-R tumor lines is inhibition of the expression of TGF-β1. Thus, the lack of expression of TGF-β1 in MPA-U tumors may be related to the acquisition of autonomous growth.
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U2 - 10.3109/07357909509011687
DO - 10.3109/07357909509011687
M3 - Article
C2 - 7874571
AN - SCOPUS:0028927483
VL - 13
SP - 173
EP - 180
JO - Cancer Investigation
JF - Cancer Investigation
SN - 0735-7907
IS - 2
ER -