Correlation of Quantitative Digital Image Analysis with the Glomerular Filtration Rate in Chronic Allograft Nephropathy

Montserrat M. Diaz Encarnacion, Matthew D. Griffin, Jeffrey M. Slezak, Erik J. Bergstralh, Mark D Stegall, Jorge A. Velosa, Joseph Peter Grande

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

Chronic allograft nephropathy (CAN) is characterized by progressive renal functional loss and histologic abnormalities of one or more tissue compartments. In this study, correlations between histologic abnormalities and graft function [glomerular filtration rate (GFR, measured by iothalamate clearance), serum creatinine (SCr) and urinary protein (UPr)] were investigated using biopsies from 49 patients with newly diagnosed CAN. Extent of tubulointerstitial fibrosis (%TIF), as assessed by a semi-quantitative score, correlated significantly with GFR, SCr and UPr. The close correlation between %TIF and GFR suggested that quantitative measurement of %TIF may predict functional consequences of CAN. Calculation of %TIF by computerized digital analysis was performed using four strategies: (a) quantitation of blue material in Masson's trichrome (MT)-stained sections, (b) quantitation of red material in Sirius Red-stained sections (SR-nonpolarized), (c) quantitation of birefringent material in Sirius Red stained-sections examined under polarized light (SR-polarized) and (d) quantification of brown material in sections stained by immunoperoxidase for alpha-smooth muscle actin. Only the SR-nonpolarized score correlated significantly with GFR at the time of biopsy-diagnosis of CAN. We conclude that digital analysis strategies demonstrate variable accuracy in quantifying %TIF. Validation of the SR-nonpolarized strategy against histologic scoring and GFR supports the application of this technique to longitudinal studies of CAN.

Original languageEnglish (US)
Pages (from-to)248-256
Number of pages9
JournalAmerican Journal of Transplantation
Volume4
Issue number2
DOIs
StatePublished - Feb 2004

Fingerprint

Glomerular Filtration Rate
Allografts
Creatinine
Iothalamic Acid
Biopsy
Serum
Smooth Muscle
Longitudinal Studies
Actins
Proteins
Fibrosis
Transplants
Kidney
Light

Keywords

  • Chronic allograft nephropathy
  • Image analysis
  • Interstitial fibrosis

ASJC Scopus subject areas

  • Immunology

Cite this

Correlation of Quantitative Digital Image Analysis with the Glomerular Filtration Rate in Chronic Allograft Nephropathy. / Diaz Encarnacion, Montserrat M.; Griffin, Matthew D.; Slezak, Jeffrey M.; Bergstralh, Erik J.; Stegall, Mark D; Velosa, Jorge A.; Grande, Joseph Peter.

In: American Journal of Transplantation, Vol. 4, No. 2, 02.2004, p. 248-256.

Research output: Contribution to journalArticle

Diaz Encarnacion, Montserrat M. ; Griffin, Matthew D. ; Slezak, Jeffrey M. ; Bergstralh, Erik J. ; Stegall, Mark D ; Velosa, Jorge A. ; Grande, Joseph Peter. / Correlation of Quantitative Digital Image Analysis with the Glomerular Filtration Rate in Chronic Allograft Nephropathy. In: American Journal of Transplantation. 2004 ; Vol. 4, No. 2. pp. 248-256.
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