Correlation of epithelial-mesenchymal transition markers with clinicopathologic parameters in adenocarcinomas and squamous cell carcinoma of the lung

Seok Hyung Kim, Jin Man Kim, Myeong Hee Shin, Chul Woung Kim, Song Mei Huang, Dong Wook Kang, Kwang Sun Suh, Eunhee S. Yi, Kyung Hee Kim

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Epithelial-mesenchymal transition (EMT) is characterized by the loss of epithelial cell junction proteins and the gain of mesenchymal markers. The aim of this study was to analyze the associations between the EMT-related markers vimentin, E-cadherin, ß-catenin, slug, snail, and twist1 and clinicopathologic parameters as well as epidermal growth factor receptor (EGFR) gene copy number and protein expression in non-small cell lung carcinoma (NSCLC). Fifty-nine squamous cell carcinomas (SCC) and 43 adenocarcinomas (AD) were immunohistochemically examined for respective EMT markers and for EGFR, using the EGFR PharmDx kit (Dako) for protein expression and automated silver enhanced in situ hybridization (SISH) for copy number. Vimentin expression in tumor epithelia was significantly higher in AD samples than in SCC samples (P=0.015). Among AD samples, vimentin expression was positively correlated with histologic grade (2 vs. 3; P=0.021) and exhibited a tendency toward a positive correlation with pTNM stage (I vs. II-IV; P=0.052). EGFR gene copy number was positively correlated with EGFR protein expression among both AD samples (P=0.008) and SCC samples (P=0.042), with EGFR protein expression being significantly higher in SCC samples compared with AD (P=0.038). Among AD samples, EGFR protein expression was associated with higher cytoplasmic expression of ß-catenin (P=0.031). Among SCC samples, EGFR protein expression was negatively correlated with nuclear expression of ß-catenin (P=0.033) but positively with nuclear slug (P=0.021). The expression pattern of EMT markers in AD suggests that vimentin is a possible immunohistochemical predictor of tumor progression.

Original languageEnglish (US)
Pages (from-to)581-591
Number of pages11
JournalHistology and Histopathology
Volume27
Issue number5
StatePublished - May 1 2012

Keywords

  • Adenocarcinoma
  • EGFR
  • Epithelial-mesenchymal transition
  • Non-small cell lung carcinoma
  • Squamous cell carcinoma

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology

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    Kim, S. H., Kim, J. M., Shin, M. H., Kim, C. W., Huang, S. M., Kang, D. W., Suh, K. S., Yi, E. S., & Kim, K. H. (2012). Correlation of epithelial-mesenchymal transition markers with clinicopathologic parameters in adenocarcinomas and squamous cell carcinoma of the lung. Histology and Histopathology, 27(5), 581-591.