Correlation of DNA ploidy with c-erbB-2 expression in preinvasive and invasive breast tumors

Daniel W Visscher, F. H. Sarkar, J. D. Crissman

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Detection of c-erbB-2 oncogene product expression by monoclonal antibody staining (avidin-biotin technique) in formalin-fixed, paraffin-embedded atypical hyperplasias (AH, n=20), intraductal carcinomas (IDCA, n=27) and invasive carcinomas (INVCA, n=48) was compared to ploidy determinations obtained by flow cytometry (INVCA) or image analysis (AH, IDCA). Cytoplasmic membrane staining was present in 11/48 (23%) INVCA and 8/27 (30%) IDCA but none of the AH. Tumors with abnormal DNA content expressed c-erbB-2 more frequently: INVCA, 2/19 (11%) diploid range versus 9/29 (31%) aneuploid; IDCA, 1/7 (14%) diploid range versus 7/20 (35%) aneuploid. Poorly differentiated (nuclear grade) IDCA or INVCA were also more frequently stained (14/35, 40%) than were well or moderately differentiated cases (5/40, 12.5%). Oncogene product expression and DNA content derangements may be related biologic parameters in breast neoplasia, and both are highly associated with cytologic nuclear abnormalities.

Original languageEnglish (US)
Pages (from-to)418-424
Number of pages7
JournalAnalytical and Quantitative Cytology and Histology
Volume13
Issue number6
StatePublished - 1991
Externally publishedYes

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Ploidies
Oncogene Proteins
Aneuploidy
Diploidy
Staining and Labeling
Breast Neoplasms
Carcinoma, Intraductal, Noninfiltrating
Avidin
DNA
Biotin
Paraffin
Formaldehyde
Hyperplasia
Neoplasms
Flow Cytometry
Breast
Monoclonal Antibodies
Cell Membrane
Carcinoma

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy
  • Histology

Cite this

Correlation of DNA ploidy with c-erbB-2 expression in preinvasive and invasive breast tumors. / Visscher, Daniel W; Sarkar, F. H.; Crissman, J. D.

In: Analytical and Quantitative Cytology and Histology, Vol. 13, No. 6, 1991, p. 418-424.

Research output: Contribution to journalArticle

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