Correlation of cytogenetic and fluorescence in situ hybridization (FISH) studies in normal and gliotic brain

Stephen J. Dalrymple, John F. Herath, Thomas J. Borell, Cheryl A. Moertel, Robert Brian Jenkins

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

In vitro tissue culturing, for karyotype analysis, may introduce artifacts confounding the cytogenetic evaluation of tissues with low baseline proliferative activity. Utilizing a panel of fluorescence in situ hybridization (FISH) probes for chromosomes 7, 8, 9, 10, 12, 17, 18, X, and Y, we compared the results of FISH analysis of non-tumorous normal (11 patients) and gliotic (10 patients) brain tissue touch preparations with those of cytogenetic evaluation performed on short-term primary cultures of the same material. We found a significant rate of apparent monosomy of chromosomes 8 and 17 by FISH analysis, with no corresponding clonal chromosomal loss detected by karyotype evaluation. These monosomy rates were significantly lower in gliotic than in normal brain tissue, and image analysis suggested that this apparent monosomy was due to interphase pairing of homologous centromere signals. Two distinct Y-chromosome signals were seen in 9.4% of nuclei by FISH, with 3 of 15 males displaying disomy Y rates over 15%. Disomy Y rates correlated approximately with age and clonal disomy Y was seen in the karyotype of one of these specimens. Karyotype analysis demonstrated loss of a sex chromosome in 6 specimens, while no sex chromosome nullisomy was detected by FISH. FISH is a valuable adjunct to the cytogenetic evaluation of tissues with low baseline proliferative activity. The differences in relative monosomy rates between normal and gliotic brain suggest that alterations in nuclear architecture and/or DNA sequence accompany the transition from normal to reactive glia.

Original languageEnglish (US)
Pages (from-to)448-456
Number of pages9
JournalJournal of Neuropathology and Experimental Neurology
Volume53
Issue number5
StatePublished - Sep 1994

Fingerprint

Fluorescence In Situ Hybridization
Cytogenetics
Monosomy
Karyotype
Brain
Chromosomes, Human, Pair 8
Sex Chromosomes
Chromosomes, Human, Pair 17
Chromosomes, Human, Pair 6
Chromosomes, Human, Pair 7
Centromere
Y Chromosome
Interphase
Touch
Neuroglia
Artifacts

Keywords

  • Cytogenetics
  • Fluorescence in situ hybridization
  • Gliosis

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Neuroscience(all)

Cite this

Correlation of cytogenetic and fluorescence in situ hybridization (FISH) studies in normal and gliotic brain. / Dalrymple, Stephen J.; Herath, John F.; Borell, Thomas J.; Moertel, Cheryl A.; Jenkins, Robert Brian.

In: Journal of Neuropathology and Experimental Neurology, Vol. 53, No. 5, 09.1994, p. 448-456.

Research output: Contribution to journalArticle

Dalrymple, Stephen J. ; Herath, John F. ; Borell, Thomas J. ; Moertel, Cheryl A. ; Jenkins, Robert Brian. / Correlation of cytogenetic and fluorescence in situ hybridization (FISH) studies in normal and gliotic brain. In: Journal of Neuropathology and Experimental Neurology. 1994 ; Vol. 53, No. 5. pp. 448-456.
@article{67237b8f0178478eafb1a088d1023896,
title = "Correlation of cytogenetic and fluorescence in situ hybridization (FISH) studies in normal and gliotic brain",
abstract = "In vitro tissue culturing, for karyotype analysis, may introduce artifacts confounding the cytogenetic evaluation of tissues with low baseline proliferative activity. Utilizing a panel of fluorescence in situ hybridization (FISH) probes for chromosomes 7, 8, 9, 10, 12, 17, 18, X, and Y, we compared the results of FISH analysis of non-tumorous normal (11 patients) and gliotic (10 patients) brain tissue touch preparations with those of cytogenetic evaluation performed on short-term primary cultures of the same material. We found a significant rate of apparent monosomy of chromosomes 8 and 17 by FISH analysis, with no corresponding clonal chromosomal loss detected by karyotype evaluation. These monosomy rates were significantly lower in gliotic than in normal brain tissue, and image analysis suggested that this apparent monosomy was due to interphase pairing of homologous centromere signals. Two distinct Y-chromosome signals were seen in 9.4{\%} of nuclei by FISH, with 3 of 15 males displaying disomy Y rates over 15{\%}. Disomy Y rates correlated approximately with age and clonal disomy Y was seen in the karyotype of one of these specimens. Karyotype analysis demonstrated loss of a sex chromosome in 6 specimens, while no sex chromosome nullisomy was detected by FISH. FISH is a valuable adjunct to the cytogenetic evaluation of tissues with low baseline proliferative activity. The differences in relative monosomy rates between normal and gliotic brain suggest that alterations in nuclear architecture and/or DNA sequence accompany the transition from normal to reactive glia.",
keywords = "Cytogenetics, Fluorescence in situ hybridization, Gliosis",
author = "Dalrymple, {Stephen J.} and Herath, {John F.} and Borell, {Thomas J.} and Moertel, {Cheryl A.} and Jenkins, {Robert Brian}",
year = "1994",
month = "9",
language = "English (US)",
volume = "53",
pages = "448--456",
journal = "American Journal of Psychotherapy",
issn = "0002-9564",
publisher = "Lippincott Williams and Wilkins",
number = "5",

}

TY - JOUR

T1 - Correlation of cytogenetic and fluorescence in situ hybridization (FISH) studies in normal and gliotic brain

AU - Dalrymple, Stephen J.

AU - Herath, John F.

AU - Borell, Thomas J.

AU - Moertel, Cheryl A.

AU - Jenkins, Robert Brian

PY - 1994/9

Y1 - 1994/9

N2 - In vitro tissue culturing, for karyotype analysis, may introduce artifacts confounding the cytogenetic evaluation of tissues with low baseline proliferative activity. Utilizing a panel of fluorescence in situ hybridization (FISH) probes for chromosomes 7, 8, 9, 10, 12, 17, 18, X, and Y, we compared the results of FISH analysis of non-tumorous normal (11 patients) and gliotic (10 patients) brain tissue touch preparations with those of cytogenetic evaluation performed on short-term primary cultures of the same material. We found a significant rate of apparent monosomy of chromosomes 8 and 17 by FISH analysis, with no corresponding clonal chromosomal loss detected by karyotype evaluation. These monosomy rates were significantly lower in gliotic than in normal brain tissue, and image analysis suggested that this apparent monosomy was due to interphase pairing of homologous centromere signals. Two distinct Y-chromosome signals were seen in 9.4% of nuclei by FISH, with 3 of 15 males displaying disomy Y rates over 15%. Disomy Y rates correlated approximately with age and clonal disomy Y was seen in the karyotype of one of these specimens. Karyotype analysis demonstrated loss of a sex chromosome in 6 specimens, while no sex chromosome nullisomy was detected by FISH. FISH is a valuable adjunct to the cytogenetic evaluation of tissues with low baseline proliferative activity. The differences in relative monosomy rates between normal and gliotic brain suggest that alterations in nuclear architecture and/or DNA sequence accompany the transition from normal to reactive glia.

AB - In vitro tissue culturing, for karyotype analysis, may introduce artifacts confounding the cytogenetic evaluation of tissues with low baseline proliferative activity. Utilizing a panel of fluorescence in situ hybridization (FISH) probes for chromosomes 7, 8, 9, 10, 12, 17, 18, X, and Y, we compared the results of FISH analysis of non-tumorous normal (11 patients) and gliotic (10 patients) brain tissue touch preparations with those of cytogenetic evaluation performed on short-term primary cultures of the same material. We found a significant rate of apparent monosomy of chromosomes 8 and 17 by FISH analysis, with no corresponding clonal chromosomal loss detected by karyotype evaluation. These monosomy rates were significantly lower in gliotic than in normal brain tissue, and image analysis suggested that this apparent monosomy was due to interphase pairing of homologous centromere signals. Two distinct Y-chromosome signals were seen in 9.4% of nuclei by FISH, with 3 of 15 males displaying disomy Y rates over 15%. Disomy Y rates correlated approximately with age and clonal disomy Y was seen in the karyotype of one of these specimens. Karyotype analysis demonstrated loss of a sex chromosome in 6 specimens, while no sex chromosome nullisomy was detected by FISH. FISH is a valuable adjunct to the cytogenetic evaluation of tissues with low baseline proliferative activity. The differences in relative monosomy rates between normal and gliotic brain suggest that alterations in nuclear architecture and/or DNA sequence accompany the transition from normal to reactive glia.

KW - Cytogenetics

KW - Fluorescence in situ hybridization

KW - Gliosis

UR - http://www.scopus.com/inward/record.url?scp=0028123072&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028123072&partnerID=8YFLogxK

M3 - Article

C2 - 8083688

AN - SCOPUS:0028123072

VL - 53

SP - 448

EP - 456

JO - American Journal of Psychotherapy

JF - American Journal of Psychotherapy

SN - 0002-9564

IS - 5

ER -