Coordination of centrosome homeostasis and DNA repair is intact in MCF-7 and disrupted in MDA-MB 231 breast cancer cells

Ilie D. Acu, Tieju Liu, Kelly Suino-Powell, Steven M. Mooney, Antonio D'Assoro, Nicholas Rowland, Alysson R. Muotri, Ricardo G. Correa, Yun Niu, Rajiv Kumar, Jeffrey L Salisbury

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

When cells encounter substantial DNA damage, critical cell cycle events are halted while DNA repair mechanisms are activated to restore genome integrity. Genomic integrity also depends on proper assembly and function of the bipolar mitotic spindle, which is required for equal chromosome segregation. Failure to execute either of these processes leads to genomic instability, aging, and cancer. Here, we show that following DNA damage in the breast cancer cell line MCF-7, the centrosome protein centrin2 moves from the cytoplasm and accumulates in the nucleus in a xeroderma pigmentosum complementation group C protein (XPC)-dependent manner, reducing the available cytoplasmic pool of this key centriole protein and preventing centrosome amplification. MDA-MB 231 cells do not express XPC and fail to move centrin into the nucleus following DNA damage. Reintroduction of XPC expression in MDA-MB 231 cells rescues nuclear centrin2 sequestration and reestablishes control against centrosome amplification, regardless of mutant p53 status. Importantly, the capacity to repair DNA damage was also dependent on the availability of centrin2 in the nucleus. These observations show that centrin and XPC cooperate in a reciprocal mechanism to coordinate centrosome homeostasis and DNA repair and suggest that this process may provide a tractable target to develop treatments to slow progression of cancer and aging.

Original languageEnglish (US)
Pages (from-to)3320-3328
Number of pages9
JournalCancer Research
Volume70
Issue number8
DOIs
StatePublished - Apr 15 2010

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Centrosome
DNA Repair
DNA Damage
Homeostasis
Breast Neoplasms
Sulfamethoxazole Drug Combination Trimethoprim
Centrioles
Chromosome Segregation
Spindle Apparatus
Proteins
Genomic Instability
Neoplasms
Cell Cycle
Cytoplasm
Genome
Cell Line

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Coordination of centrosome homeostasis and DNA repair is intact in MCF-7 and disrupted in MDA-MB 231 breast cancer cells. / Acu, Ilie D.; Liu, Tieju; Suino-Powell, Kelly; Mooney, Steven M.; D'Assoro, Antonio; Rowland, Nicholas; Muotri, Alysson R.; Correa, Ricardo G.; Niu, Yun; Kumar, Rajiv; Salisbury, Jeffrey L.

In: Cancer Research, Vol. 70, No. 8, 15.04.2010, p. 3320-3328.

Research output: Contribution to journalArticle

Acu, Ilie D. ; Liu, Tieju ; Suino-Powell, Kelly ; Mooney, Steven M. ; D'Assoro, Antonio ; Rowland, Nicholas ; Muotri, Alysson R. ; Correa, Ricardo G. ; Niu, Yun ; Kumar, Rajiv ; Salisbury, Jeffrey L. / Coordination of centrosome homeostasis and DNA repair is intact in MCF-7 and disrupted in MDA-MB 231 breast cancer cells. In: Cancer Research. 2010 ; Vol. 70, No. 8. pp. 3320-3328.
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