Coordinated, diurnal hexose transporter expression in rat small bowel: Implications for small bowel resection

Scott G. Houghton, Corey W. Iqbal, Judith A. Duenes, Javairiah Fatima, Michael S. Kasparek, Michael G. Sarr

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Background: Hexose transporter mRNA and protein levels follow a diurnal rhythm in rat jejunum. Their coordinated expression and resultant function throughout the small bowel is not well understood. We hypothesized that hexose transporter levels and glucose absorption follow a coordinated, site-specific diurnal rhythm in rat duodenum and jejunum, but not in ileum. Methods: Sprague-Dawley rats were housed in a strictly maintained, 12-h, light/dark room [light 6 am to 6 pm] with free access to water and chow. Mucosa was harvested from duodenum, jejunum, and ileum at 3 am, 9 am, 3 pm, and 9 pm, and full thickness 1-cm segments were harvested at 9 am, and 9 pm (n = 6 for each segment at each time point). mRNA levels were determined by reverse-transcription, real-time polymerase chain reaction (n ≥ 5), protein levels by semiquantitative Western blotting (n ≥ 5), and transporter-mediated glucose uptake by everted sleeve technique (n = 6). Results: mRNA levels of SGLT1 and GLUT5 followed a temporally coordinated, diurnal rhythm in all 3 segments (P < .01), while mRNA for GLUT2 and protein levels for SGLT1 and GLUT2 varied diurnally only in duodenum and jejunum (P > .05) but not in ileum (P > .10). SGLT1 and GLUT5 mRNA induction decreased aborally. Baseline SGLT1 and GLUT5 mRNA levels and SLGT1 and GLUT2 protein levels did not vary aborally (P > .05 for all). GLUT2 mRNA baseline levels were decreased in ileum (P < .01). Glucose uptake varied diurnally in duodenum and jejunum with no difference in ileum. Transporter-mediated glucose uptake was greater in duodenum and jejunum compared with ileum. Conclusion: Regulation of hexose absorption in rat small bowel seems to be site-specific and mediated by multiple mechanisms.

Original languageEnglish (US)
Pages (from-to)79-93
Number of pages15
JournalSurgery
Volume143
Issue number1
DOIs
StatePublished - Jan 2008

Fingerprint

Monosaccharide Transport Proteins
Ileum
Jejunum
Duodenum
Messenger RNA
Circadian Rhythm
Facilitative Glucose Transport Proteins
Glucose Transporter Type 2
Light
Glucose
Hexoses
Reverse Transcription
Sprague Dawley Rats
Real-Time Polymerase Chain Reaction
Mucous Membrane
Proteins
Western Blotting
Water

ASJC Scopus subject areas

  • Surgery

Cite this

Houghton, S. G., Iqbal, C. W., Duenes, J. A., Fatima, J., Kasparek, M. S., & Sarr, M. G. (2008). Coordinated, diurnal hexose transporter expression in rat small bowel: Implications for small bowel resection. Surgery, 143(1), 79-93. https://doi.org/10.1016/j.surg.2007.06.007

Coordinated, diurnal hexose transporter expression in rat small bowel : Implications for small bowel resection. / Houghton, Scott G.; Iqbal, Corey W.; Duenes, Judith A.; Fatima, Javairiah; Kasparek, Michael S.; Sarr, Michael G.

In: Surgery, Vol. 143, No. 1, 01.2008, p. 79-93.

Research output: Contribution to journalArticle

Houghton, SG, Iqbal, CW, Duenes, JA, Fatima, J, Kasparek, MS & Sarr, MG 2008, 'Coordinated, diurnal hexose transporter expression in rat small bowel: Implications for small bowel resection', Surgery, vol. 143, no. 1, pp. 79-93. https://doi.org/10.1016/j.surg.2007.06.007
Houghton, Scott G. ; Iqbal, Corey W. ; Duenes, Judith A. ; Fatima, Javairiah ; Kasparek, Michael S. ; Sarr, Michael G. / Coordinated, diurnal hexose transporter expression in rat small bowel : Implications for small bowel resection. In: Surgery. 2008 ; Vol. 143, No. 1. pp. 79-93.
@article{ecb8bbc7fa9248859e452b0d66e3f5e3,
title = "Coordinated, diurnal hexose transporter expression in rat small bowel: Implications for small bowel resection",
abstract = "Background: Hexose transporter mRNA and protein levels follow a diurnal rhythm in rat jejunum. Their coordinated expression and resultant function throughout the small bowel is not well understood. We hypothesized that hexose transporter levels and glucose absorption follow a coordinated, site-specific diurnal rhythm in rat duodenum and jejunum, but not in ileum. Methods: Sprague-Dawley rats were housed in a strictly maintained, 12-h, light/dark room [light 6 am to 6 pm] with free access to water and chow. Mucosa was harvested from duodenum, jejunum, and ileum at 3 am, 9 am, 3 pm, and 9 pm, and full thickness 1-cm segments were harvested at 9 am, and 9 pm (n = 6 for each segment at each time point). mRNA levels were determined by reverse-transcription, real-time polymerase chain reaction (n ≥ 5), protein levels by semiquantitative Western blotting (n ≥ 5), and transporter-mediated glucose uptake by everted sleeve technique (n = 6). Results: mRNA levels of SGLT1 and GLUT5 followed a temporally coordinated, diurnal rhythm in all 3 segments (P < .01), while mRNA for GLUT2 and protein levels for SGLT1 and GLUT2 varied diurnally only in duodenum and jejunum (P > .05) but not in ileum (P > .10). SGLT1 and GLUT5 mRNA induction decreased aborally. Baseline SGLT1 and GLUT5 mRNA levels and SLGT1 and GLUT2 protein levels did not vary aborally (P > .05 for all). GLUT2 mRNA baseline levels were decreased in ileum (P < .01). Glucose uptake varied diurnally in duodenum and jejunum with no difference in ileum. Transporter-mediated glucose uptake was greater in duodenum and jejunum compared with ileum. Conclusion: Regulation of hexose absorption in rat small bowel seems to be site-specific and mediated by multiple mechanisms.",
author = "Houghton, {Scott G.} and Iqbal, {Corey W.} and Duenes, {Judith A.} and Javairiah Fatima and Kasparek, {Michael S.} and Sarr, {Michael G.}",
year = "2008",
month = "1",
doi = "10.1016/j.surg.2007.06.007",
language = "English (US)",
volume = "143",
pages = "79--93",
journal = "Surgery (United States)",
issn = "0039-6060",
publisher = "Mosby Inc.",
number = "1",

}

TY - JOUR

T1 - Coordinated, diurnal hexose transporter expression in rat small bowel

T2 - Implications for small bowel resection

AU - Houghton, Scott G.

AU - Iqbal, Corey W.

AU - Duenes, Judith A.

AU - Fatima, Javairiah

AU - Kasparek, Michael S.

AU - Sarr, Michael G.

PY - 2008/1

Y1 - 2008/1

N2 - Background: Hexose transporter mRNA and protein levels follow a diurnal rhythm in rat jejunum. Their coordinated expression and resultant function throughout the small bowel is not well understood. We hypothesized that hexose transporter levels and glucose absorption follow a coordinated, site-specific diurnal rhythm in rat duodenum and jejunum, but not in ileum. Methods: Sprague-Dawley rats were housed in a strictly maintained, 12-h, light/dark room [light 6 am to 6 pm] with free access to water and chow. Mucosa was harvested from duodenum, jejunum, and ileum at 3 am, 9 am, 3 pm, and 9 pm, and full thickness 1-cm segments were harvested at 9 am, and 9 pm (n = 6 for each segment at each time point). mRNA levels were determined by reverse-transcription, real-time polymerase chain reaction (n ≥ 5), protein levels by semiquantitative Western blotting (n ≥ 5), and transporter-mediated glucose uptake by everted sleeve technique (n = 6). Results: mRNA levels of SGLT1 and GLUT5 followed a temporally coordinated, diurnal rhythm in all 3 segments (P < .01), while mRNA for GLUT2 and protein levels for SGLT1 and GLUT2 varied diurnally only in duodenum and jejunum (P > .05) but not in ileum (P > .10). SGLT1 and GLUT5 mRNA induction decreased aborally. Baseline SGLT1 and GLUT5 mRNA levels and SLGT1 and GLUT2 protein levels did not vary aborally (P > .05 for all). GLUT2 mRNA baseline levels were decreased in ileum (P < .01). Glucose uptake varied diurnally in duodenum and jejunum with no difference in ileum. Transporter-mediated glucose uptake was greater in duodenum and jejunum compared with ileum. Conclusion: Regulation of hexose absorption in rat small bowel seems to be site-specific and mediated by multiple mechanisms.

AB - Background: Hexose transporter mRNA and protein levels follow a diurnal rhythm in rat jejunum. Their coordinated expression and resultant function throughout the small bowel is not well understood. We hypothesized that hexose transporter levels and glucose absorption follow a coordinated, site-specific diurnal rhythm in rat duodenum and jejunum, but not in ileum. Methods: Sprague-Dawley rats were housed in a strictly maintained, 12-h, light/dark room [light 6 am to 6 pm] with free access to water and chow. Mucosa was harvested from duodenum, jejunum, and ileum at 3 am, 9 am, 3 pm, and 9 pm, and full thickness 1-cm segments were harvested at 9 am, and 9 pm (n = 6 for each segment at each time point). mRNA levels were determined by reverse-transcription, real-time polymerase chain reaction (n ≥ 5), protein levels by semiquantitative Western blotting (n ≥ 5), and transporter-mediated glucose uptake by everted sleeve technique (n = 6). Results: mRNA levels of SGLT1 and GLUT5 followed a temporally coordinated, diurnal rhythm in all 3 segments (P < .01), while mRNA for GLUT2 and protein levels for SGLT1 and GLUT2 varied diurnally only in duodenum and jejunum (P > .05) but not in ileum (P > .10). SGLT1 and GLUT5 mRNA induction decreased aborally. Baseline SGLT1 and GLUT5 mRNA levels and SLGT1 and GLUT2 protein levels did not vary aborally (P > .05 for all). GLUT2 mRNA baseline levels were decreased in ileum (P < .01). Glucose uptake varied diurnally in duodenum and jejunum with no difference in ileum. Transporter-mediated glucose uptake was greater in duodenum and jejunum compared with ileum. Conclusion: Regulation of hexose absorption in rat small bowel seems to be site-specific and mediated by multiple mechanisms.

UR - http://www.scopus.com/inward/record.url?scp=37249085153&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=37249085153&partnerID=8YFLogxK

U2 - 10.1016/j.surg.2007.06.007

DO - 10.1016/j.surg.2007.06.007

M3 - Article

C2 - 18154936

AN - SCOPUS:37249085153

VL - 143

SP - 79

EP - 93

JO - Surgery (United States)

JF - Surgery (United States)

SN - 0039-6060

IS - 1

ER -