Coordinate transcriptional regulation of the three fibrinogen subunit genes by glucocorticoids in cultured primary liver cells from Xenopus laevis

Lewis Rowland Roberts, Lene J. Holland

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Xenopus laevis primary hepatocytes in culture are induced by glucocorticoid hormones to synthesize and secrete fibrinogen. The increase in production of the protein is preceded by a 10- to 30-fold elevation of the mRNAs coding for the three fibrinogen subunits, Aα, Bβ, and γ. To analyze the mechanisms underlying this coordinate control of independent genes in a common regulatory network, we show here that the steroid hormone induced simultaneous activation of transcription of the three fibrinogen subunit genes. Using an optimized transcription run-on assay for nuclei from Xenopus primary liver cells, we demonstrate that glucocorticoids rapidly stimulated transcription of the Aα fibrinogen subunit gene by 15- to 20-fold, the Bβ gene by 5- to 10-fold, and the γ gene by 5- to 15-fold. The three genes exhibited a highly concerted response to the hormone, in which maximal stimulation occurred by 30 min and was maintained for at least 16 h. Blocking new protein synthesis before hormone treatment reduced total transcription by 45% and partially inhibited specific hormonal induction of all three fibrinogen subunit genes. The effect of glucocorticoids on fibrinogen transcription, therefore, was dependent in part on ongoing protein synthesis, suggesting that hormonal stimulation uses already synthesized stable factors, but also requires labile or newly synthesized factors for the full effect.

Original languageEnglish (US)
Pages (from-to)2563-2570
Number of pages8
JournalEndocrinology
Volume132
Issue number6
StatePublished - Jun 1993

Fingerprint

Xenopus laevis
Fibrinogen
Glucocorticoids
Liver
Genes
Hormones
Proteins
Xenopus
Transcriptional Activation
Hepatocytes
Steroids
Messenger RNA

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

@article{cdd14ff311694c39a4abc84da9234b7f,
title = "Coordinate transcriptional regulation of the three fibrinogen subunit genes by glucocorticoids in cultured primary liver cells from Xenopus laevis",
abstract = "Xenopus laevis primary hepatocytes in culture are induced by glucocorticoid hormones to synthesize and secrete fibrinogen. The increase in production of the protein is preceded by a 10- to 30-fold elevation of the mRNAs coding for the three fibrinogen subunits, Aα, Bβ, and γ. To analyze the mechanisms underlying this coordinate control of independent genes in a common regulatory network, we show here that the steroid hormone induced simultaneous activation of transcription of the three fibrinogen subunit genes. Using an optimized transcription run-on assay for nuclei from Xenopus primary liver cells, we demonstrate that glucocorticoids rapidly stimulated transcription of the Aα fibrinogen subunit gene by 15- to 20-fold, the Bβ gene by 5- to 10-fold, and the γ gene by 5- to 15-fold. The three genes exhibited a highly concerted response to the hormone, in which maximal stimulation occurred by 30 min and was maintained for at least 16 h. Blocking new protein synthesis before hormone treatment reduced total transcription by 45{\%} and partially inhibited specific hormonal induction of all three fibrinogen subunit genes. The effect of glucocorticoids on fibrinogen transcription, therefore, was dependent in part on ongoing protein synthesis, suggesting that hormonal stimulation uses already synthesized stable factors, but also requires labile or newly synthesized factors for the full effect.",
author = "Roberts, {Lewis Rowland} and Holland, {Lene J.}",
year = "1993",
month = "6",
language = "English (US)",
volume = "132",
pages = "2563--2570",
journal = "Endocrinology",
issn = "0013-7227",
publisher = "The Endocrine Society",
number = "6",

}

TY - JOUR

T1 - Coordinate transcriptional regulation of the three fibrinogen subunit genes by glucocorticoids in cultured primary liver cells from Xenopus laevis

AU - Roberts, Lewis Rowland

AU - Holland, Lene J.

PY - 1993/6

Y1 - 1993/6

N2 - Xenopus laevis primary hepatocytes in culture are induced by glucocorticoid hormones to synthesize and secrete fibrinogen. The increase in production of the protein is preceded by a 10- to 30-fold elevation of the mRNAs coding for the three fibrinogen subunits, Aα, Bβ, and γ. To analyze the mechanisms underlying this coordinate control of independent genes in a common regulatory network, we show here that the steroid hormone induced simultaneous activation of transcription of the three fibrinogen subunit genes. Using an optimized transcription run-on assay for nuclei from Xenopus primary liver cells, we demonstrate that glucocorticoids rapidly stimulated transcription of the Aα fibrinogen subunit gene by 15- to 20-fold, the Bβ gene by 5- to 10-fold, and the γ gene by 5- to 15-fold. The three genes exhibited a highly concerted response to the hormone, in which maximal stimulation occurred by 30 min and was maintained for at least 16 h. Blocking new protein synthesis before hormone treatment reduced total transcription by 45% and partially inhibited specific hormonal induction of all three fibrinogen subunit genes. The effect of glucocorticoids on fibrinogen transcription, therefore, was dependent in part on ongoing protein synthesis, suggesting that hormonal stimulation uses already synthesized stable factors, but also requires labile or newly synthesized factors for the full effect.

AB - Xenopus laevis primary hepatocytes in culture are induced by glucocorticoid hormones to synthesize and secrete fibrinogen. The increase in production of the protein is preceded by a 10- to 30-fold elevation of the mRNAs coding for the three fibrinogen subunits, Aα, Bβ, and γ. To analyze the mechanisms underlying this coordinate control of independent genes in a common regulatory network, we show here that the steroid hormone induced simultaneous activation of transcription of the three fibrinogen subunit genes. Using an optimized transcription run-on assay for nuclei from Xenopus primary liver cells, we demonstrate that glucocorticoids rapidly stimulated transcription of the Aα fibrinogen subunit gene by 15- to 20-fold, the Bβ gene by 5- to 10-fold, and the γ gene by 5- to 15-fold. The three genes exhibited a highly concerted response to the hormone, in which maximal stimulation occurred by 30 min and was maintained for at least 16 h. Blocking new protein synthesis before hormone treatment reduced total transcription by 45% and partially inhibited specific hormonal induction of all three fibrinogen subunit genes. The effect of glucocorticoids on fibrinogen transcription, therefore, was dependent in part on ongoing protein synthesis, suggesting that hormonal stimulation uses already synthesized stable factors, but also requires labile or newly synthesized factors for the full effect.

UR - http://www.scopus.com/inward/record.url?scp=0027195150&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027195150&partnerID=8YFLogxK

M3 - Article

C2 - 8504758

AN - SCOPUS:0027195150

VL - 132

SP - 2563

EP - 2570

JO - Endocrinology

JF - Endocrinology

SN - 0013-7227

IS - 6

ER -