Coordinate secretion of acid hydrolases in rat bile. Hepatocyte exocytosis of lysosomal protein?

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Abstract

Three lysosomal glycosidases, β-glucuronidase (EC 3.2.1.31), β-galactosidase (EC 3.2.1.23), and N-acetyl-β-glucosaminidase (EC 3.2.1.30) have been investigated in bile that was freshly collected from rats through a complete bile fistula. Assay conditions have been established on the basis of appropriate kinetic studies. The biliary excretion patterns for these enzymes were found to vary considerably from rat to rat during the 24-hr collection period. In a given animal, however, the three hydrolases were excreted in parallel and showed a gradual increase in activity with time, most marked after 10-12 hr of collection. 24-hr biliary outputs of the three hydrolases averaged ≃ 3% of their respective contents in total liver, and bile diversion had no effect on hepatic glycosidase activity or total protein content. Other enzymes known to be associated primarily with mitochondria, endoplasmic reticulum, and cell sap were also detected in bile, generally in smaller amounts. The biliary excretion of the plasma membrane markers, alkaline phosphodiesterase I and 5'-nucleotidase, however, was comparable to that of the lysosomal hydrolases. Biliary excretion of total protein was relatively constant and corresponded to 3.0% of the total hepatic protein content per day, whereas biliary bile acid secretion decreased during the first 12 hr and then remained constant. Exocytic bulk discharge of hepatocyte lysosomes is proposed as the most likely mechanism for the biliary excretion of lysosomal enzymes. These results call attention to the possible pathophysiologic significance of biliary excretion of hepatic lysosomal contents as a means of residue disposal.

Original languageEnglish (US)
Pages (from-to)948-954
Number of pages7
JournalJournal of Clinical Investigation
Volume64
Issue number4
StatePublished - 1979
Externally publishedYes

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Exocytosis
Hydrolases
Bile
Hepatocytes
Acids
Glycoside Hydrolases
Liver
Enzymes
Phosphodiesterase I
Galactosidases
Type 5 Cyclic Nucleotide Phosphodiesterases
Hexosaminidases
5'-Nucleotidase
Proteins
Glucuronidase
Lysosomes
Bile Acids and Salts
Endoplasmic Reticulum
Fistula
Mitochondria

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Coordinate secretion of acid hydrolases in rat bile. Hepatocyte exocytosis of lysosomal protein? / La Russo, Nicholas F; Fowler, S.

In: Journal of Clinical Investigation, Vol. 64, No. 4, 1979, p. 948-954.

Research output: Contribution to journalArticle

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abstract = "Three lysosomal glycosidases, β-glucuronidase (EC 3.2.1.31), β-galactosidase (EC 3.2.1.23), and N-acetyl-β-glucosaminidase (EC 3.2.1.30) have been investigated in bile that was freshly collected from rats through a complete bile fistula. Assay conditions have been established on the basis of appropriate kinetic studies. The biliary excretion patterns for these enzymes were found to vary considerably from rat to rat during the 24-hr collection period. In a given animal, however, the three hydrolases were excreted in parallel and showed a gradual increase in activity with time, most marked after 10-12 hr of collection. 24-hr biliary outputs of the three hydrolases averaged ≃ 3{\%} of their respective contents in total liver, and bile diversion had no effect on hepatic glycosidase activity or total protein content. Other enzymes known to be associated primarily with mitochondria, endoplasmic reticulum, and cell sap were also detected in bile, generally in smaller amounts. The biliary excretion of the plasma membrane markers, alkaline phosphodiesterase I and 5'-nucleotidase, however, was comparable to that of the lysosomal hydrolases. Biliary excretion of total protein was relatively constant and corresponded to 3.0{\%} of the total hepatic protein content per day, whereas biliary bile acid secretion decreased during the first 12 hr and then remained constant. Exocytic bulk discharge of hepatocyte lysosomes is proposed as the most likely mechanism for the biliary excretion of lysosomal enzymes. These results call attention to the possible pathophysiologic significance of biliary excretion of hepatic lysosomal contents as a means of residue disposal.",
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T1 - Coordinate secretion of acid hydrolases in rat bile. Hepatocyte exocytosis of lysosomal protein?

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AU - Fowler, S.

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