Controlling dispersion of axonal regeneration using a multichannel collagen nerve conduit

Li Yao, Godard C W de Ruiter, Huan D Wang, Andrew M. Knight, Robert J. Spinner, Michael J Yaszemski, Anthony John Windebank, Abhay Pandit

Research output: Contribution to journalArticle

122 Citations (Scopus)

Abstract

Single channel conduits are used clinically in nerve repair as an alternative to the autologous nerve graft. Axons regenerating across single channel tubes, however, may disperse resulting in inappropriate target reinnervation. This dispersion may be limited by multichannel nerve conduits as they resemble the structure of nerve multiple basal lamina tubes. In this study, we investigated the influence of channel number on the axonal regeneration using a series of 1-, 2-, 4-, and 7-channel collagen conduits and commercial (NeuraGen. ®) single channel conduits. Nerve conduits were implanted in rats with a 1 cm gap of sciatic nerve. After four months, quantitative results of regeneration were evaluated with nerve morphometry and the accuracy of regeneration was assessed using retrograde tracing: two tracers being applied simultaneously to tibial and peroneal nerves to determine the percentage of motor neurons with double projections. Recovery of function was investigated with compound muscle action potential recordings and ankle motion analysis. We showed that the fabricated 1-channel and 4-channel conduits are superior to other types of conduits in axonal regeneration. Simultaneous tracing showed a significantly lower percentage of motor neurons with double projections after 2- and 4-channel compared with 1-channel conduit repair. This study shows the potential influence of multichannel guidance on limiting dispersion without decreasing quantitative results of regeneration.

Original languageEnglish (US)
Pages (from-to)5789-5797
Number of pages9
JournalBiomaterials
Volume31
Issue number22
DOIs
StatePublished - Aug 2010

Fingerprint

Collagen
Neurons
Regeneration
Repair
Grafts
Muscle
Rats
Motor Neurons
Recovery
Tibial Nerve
Peroneal Nerve
Recovery of Function
Sciatic Nerve
Basement Membrane
Ankle
Action Potentials
Axons
Transplants
Muscles
Motion analysis

Keywords

  • Collagen
  • In vivo
  • Nerve conduit
  • Nerve regeneration
  • Neural tissue engineering

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

Controlling dispersion of axonal regeneration using a multichannel collagen nerve conduit. / Yao, Li; de Ruiter, Godard C W; Wang, Huan D; Knight, Andrew M.; Spinner, Robert J.; Yaszemski, Michael J; Windebank, Anthony John; Pandit, Abhay.

In: Biomaterials, Vol. 31, No. 22, 08.2010, p. 5789-5797.

Research output: Contribution to journalArticle

Yao, Li ; de Ruiter, Godard C W ; Wang, Huan D ; Knight, Andrew M. ; Spinner, Robert J. ; Yaszemski, Michael J ; Windebank, Anthony John ; Pandit, Abhay. / Controlling dispersion of axonal regeneration using a multichannel collagen nerve conduit. In: Biomaterials. 2010 ; Vol. 31, No. 22. pp. 5789-5797.
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