Controlled delivery of vancomycin via charged hydrogels

Carl T. Gustafson, Felix Boakye-Agyeman, Cassandra L. Brinkman, Joel M Reid, Robin Patel, Zeljko Bajzer, Mahrokh Dadsetan, Michael J Yaszemski

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Surgical site infection (SSI) remains a significant risk for any clean orthopedic surgical procedure. Complications resulting from an SSI often require a second surgery and lengthen patient recovery time. The efficacy of antimicrobial agents delivered to combat SSI is diminished by systemic toxicity, bacterial resistance, and patient compliance to dosing schedules. We submit that development of localized, controlled release formulations for antimicrobial compounds would improve the effectiveness of prophylactic surgical wound antibiotic treatment while decreasing systemic side effects. Our research group developed and characterized oligo(poly(ethylene glycol)fumarate)/sodium methacrylate (OPF/SMA) charged copolymers as biocompatible hydrogel matrices. Here, we report the engineering of this copolymer for use as an antibiotic delivery vehicle in surgical applications. We demonstrate that these hydrogels can be efficiently loaded with vancomycin (over 500 μg drug per mg hydrogel) and this loading mechanism is both time- and charge-dependent. Vancomycin release kinetics are shown to be dependent on copolymer negative charge. In the first 6 hours, we achieved as low as 33.7% release. In the first 24 hours, under 80% of total loaded drug was released. Further, vancomycin release from this system can be extended past four days. Finally, we show that the antimicrobial activity of released vancomycin is equivalent to stock vancomycin in inhibiting the growth of colonies of a clinically derived strain of methicillin-resistant Staphylococcus aureus. In summary, our work demonstrates that OPF/SMA hydrogels are appropriate candidates to deliver local antibiotic therapy for prophylaxis of surgical site infection.

Original languageEnglish (US)
Article numbere0146401
JournalPLoS One
Volume11
Issue number1
DOIs
StatePublished - Jan 1 2016

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Hydrogels
vancomycin
hydrocolloids
Vancomycin
Surgical Wound Infection
composite polymers
fumaric acid
Copolymers
anti-infective agents
Hydrogel
antibiotics
Anti-Bacterial Agents
polyethylene glycol
infection
surgery
patient compliance
Orthopedic Procedures
drugs
Methicillin
Antibiotic Prophylaxis

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Controlled delivery of vancomycin via charged hydrogels. / Gustafson, Carl T.; Boakye-Agyeman, Felix; Brinkman, Cassandra L.; Reid, Joel M; Patel, Robin; Bajzer, Zeljko; Dadsetan, Mahrokh; Yaszemski, Michael J.

In: PLoS One, Vol. 11, No. 1, e0146401, 01.01.2016.

Research output: Contribution to journalArticle

Gustafson, CT, Boakye-Agyeman, F, Brinkman, CL, Reid, JM, Patel, R, Bajzer, Z, Dadsetan, M & Yaszemski, MJ 2016, 'Controlled delivery of vancomycin via charged hydrogels', PLoS One, vol. 11, no. 1, e0146401. https://doi.org/10.1371/journal.pone.0146401
Gustafson CT, Boakye-Agyeman F, Brinkman CL, Reid JM, Patel R, Bajzer Z et al. Controlled delivery of vancomycin via charged hydrogels. PLoS One. 2016 Jan 1;11(1). e0146401. https://doi.org/10.1371/journal.pone.0146401
Gustafson, Carl T. ; Boakye-Agyeman, Felix ; Brinkman, Cassandra L. ; Reid, Joel M ; Patel, Robin ; Bajzer, Zeljko ; Dadsetan, Mahrokh ; Yaszemski, Michael J. / Controlled delivery of vancomycin via charged hydrogels. In: PLoS One. 2016 ; Vol. 11, No. 1.
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