Control of maintenance and regeneration of planarian eyes by ovo

Samuel D. Cross, Adiv A. Johnson, Benjamin J. Gilles, Lori A. Bachman, Takeshi Inoue, Kiyokazu Agata, Lihua Y Marmorstein, Alan D Marmorstein

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

PURPOSE. Following decapitation, the planarian Schmidtea mediterranea regenerates its head and eyes. The gene ovo is required for eye maintenance and regeneration in response to wounding. In this study, we investigated whether eye regeneration in S. mediterranea could occur absent a wound healing response. METHODS. One hundred twenty S. mediterranea were treated with ovo RNA interference (RNAi) or control (unc-22) RNAi by feeding double-stranded RNA (dsRNA). Following eye loss, ovo RNAi treatment was halted and replaced with control RNAi treatment. Quantitative real-time PCR (qPCR) was used to monitor ovo expression. Eye functionality was monitored via a phototaxis assay. Photoreceptor neurons were visualized via immunofluorescence staining of arrestin. RESULTS. Treatment with ovo RNAi caused eyes to gradually shrink until they were completely absent. One hundred percent of ovo RNAi-treated planarians lost both eyes within 137 days of treatment onset. ovo RNAi-treated planarians were unable to regenerate eyes in response to decapitation. Upon removal of ovo RNAi, eyes became visible as small pigmented spots in the head within 28 days. The eyes slowly developed, appearing to gain pigmented cells first and then nonpigmented photoreceptors. Phototaxis assays demonstrated functional eye loss and eye restoration. ovo mRNA was significantly decreased following treatment with ovo RNAi and significantly increased following removal of ovo RNAi. Arrestin staining was present in the eyes, optic nerves, and optic chiasm of worms with regenerated eyes but not in eyeless worms. CONCLUSIONS. S. mediterranea have the ability to generate functional eyes in the absence of a wound healing response. This ability requires the expression of ovo.

Original languageEnglish (US)
Pages (from-to)7604-7610
Number of pages7
JournalInvestigative Ophthalmology and Visual Science
Volume56
Issue number12
DOIs
StatePublished - Nov 1 2015

Fingerprint

Planarians
Regeneration
Maintenance
RNA Interference
Arrestin
Decapitation
Aptitude
Wound Healing
Head
Staining and Labeling
Optic Chiasm
Therapeutics
Double-Stranded RNA

Keywords

  • Ovo
  • Phototaxis
  • Planarian
  • Regeneration
  • Schmidtea mediterranea

ASJC Scopus subject areas

  • Medicine(all)
  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Control of maintenance and regeneration of planarian eyes by ovo. / Cross, Samuel D.; Johnson, Adiv A.; Gilles, Benjamin J.; Bachman, Lori A.; Inoue, Takeshi; Agata, Kiyokazu; Marmorstein, Lihua Y; Marmorstein, Alan D.

In: Investigative Ophthalmology and Visual Science, Vol. 56, No. 12, 01.11.2015, p. 7604-7610.

Research output: Contribution to journalArticle

Cross, SD, Johnson, AA, Gilles, BJ, Bachman, LA, Inoue, T, Agata, K, Marmorstein, LY & Marmorstein, AD 2015, 'Control of maintenance and regeneration of planarian eyes by ovo', Investigative Ophthalmology and Visual Science, vol. 56, no. 12, pp. 7604-7610. https://doi.org/10.1167/iovs.15-17458
Cross SD, Johnson AA, Gilles BJ, Bachman LA, Inoue T, Agata K et al. Control of maintenance and regeneration of planarian eyes by ovo. Investigative Ophthalmology and Visual Science. 2015 Nov 1;56(12):7604-7610. https://doi.org/10.1167/iovs.15-17458
Cross, Samuel D. ; Johnson, Adiv A. ; Gilles, Benjamin J. ; Bachman, Lori A. ; Inoue, Takeshi ; Agata, Kiyokazu ; Marmorstein, Lihua Y ; Marmorstein, Alan D. / Control of maintenance and regeneration of planarian eyes by ovo. In: Investigative Ophthalmology and Visual Science. 2015 ; Vol. 56, No. 12. pp. 7604-7610.
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abstract = "PURPOSE. Following decapitation, the planarian Schmidtea mediterranea regenerates its head and eyes. The gene ovo is required for eye maintenance and regeneration in response to wounding. In this study, we investigated whether eye regeneration in S. mediterranea could occur absent a wound healing response. METHODS. One hundred twenty S. mediterranea were treated with ovo RNA interference (RNAi) or control (unc-22) RNAi by feeding double-stranded RNA (dsRNA). Following eye loss, ovo RNAi treatment was halted and replaced with control RNAi treatment. Quantitative real-time PCR (qPCR) was used to monitor ovo expression. Eye functionality was monitored via a phototaxis assay. Photoreceptor neurons were visualized via immunofluorescence staining of arrestin. RESULTS. Treatment with ovo RNAi caused eyes to gradually shrink until they were completely absent. One hundred percent of ovo RNAi-treated planarians lost both eyes within 137 days of treatment onset. ovo RNAi-treated planarians were unable to regenerate eyes in response to decapitation. Upon removal of ovo RNAi, eyes became visible as small pigmented spots in the head within 28 days. The eyes slowly developed, appearing to gain pigmented cells first and then nonpigmented photoreceptors. Phototaxis assays demonstrated functional eye loss and eye restoration. ovo mRNA was significantly decreased following treatment with ovo RNAi and significantly increased following removal of ovo RNAi. Arrestin staining was present in the eyes, optic nerves, and optic chiasm of worms with regenerated eyes but not in eyeless worms. CONCLUSIONS. S. mediterranea have the ability to generate functional eyes in the absence of a wound healing response. This ability requires the expression of ovo.",
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T1 - Control of maintenance and regeneration of planarian eyes by ovo

AU - Cross, Samuel D.

AU - Johnson, Adiv A.

AU - Gilles, Benjamin J.

AU - Bachman, Lori A.

AU - Inoue, Takeshi

AU - Agata, Kiyokazu

AU - Marmorstein, Lihua Y

AU - Marmorstein, Alan D

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Y1 - 2015/11/1

N2 - PURPOSE. Following decapitation, the planarian Schmidtea mediterranea regenerates its head and eyes. The gene ovo is required for eye maintenance and regeneration in response to wounding. In this study, we investigated whether eye regeneration in S. mediterranea could occur absent a wound healing response. METHODS. One hundred twenty S. mediterranea were treated with ovo RNA interference (RNAi) or control (unc-22) RNAi by feeding double-stranded RNA (dsRNA). Following eye loss, ovo RNAi treatment was halted and replaced with control RNAi treatment. Quantitative real-time PCR (qPCR) was used to monitor ovo expression. Eye functionality was monitored via a phototaxis assay. Photoreceptor neurons were visualized via immunofluorescence staining of arrestin. RESULTS. Treatment with ovo RNAi caused eyes to gradually shrink until they were completely absent. One hundred percent of ovo RNAi-treated planarians lost both eyes within 137 days of treatment onset. ovo RNAi-treated planarians were unable to regenerate eyes in response to decapitation. Upon removal of ovo RNAi, eyes became visible as small pigmented spots in the head within 28 days. The eyes slowly developed, appearing to gain pigmented cells first and then nonpigmented photoreceptors. Phototaxis assays demonstrated functional eye loss and eye restoration. ovo mRNA was significantly decreased following treatment with ovo RNAi and significantly increased following removal of ovo RNAi. Arrestin staining was present in the eyes, optic nerves, and optic chiasm of worms with regenerated eyes but not in eyeless worms. CONCLUSIONS. S. mediterranea have the ability to generate functional eyes in the absence of a wound healing response. This ability requires the expression of ovo.

AB - PURPOSE. Following decapitation, the planarian Schmidtea mediterranea regenerates its head and eyes. The gene ovo is required for eye maintenance and regeneration in response to wounding. In this study, we investigated whether eye regeneration in S. mediterranea could occur absent a wound healing response. METHODS. One hundred twenty S. mediterranea were treated with ovo RNA interference (RNAi) or control (unc-22) RNAi by feeding double-stranded RNA (dsRNA). Following eye loss, ovo RNAi treatment was halted and replaced with control RNAi treatment. Quantitative real-time PCR (qPCR) was used to monitor ovo expression. Eye functionality was monitored via a phototaxis assay. Photoreceptor neurons were visualized via immunofluorescence staining of arrestin. RESULTS. Treatment with ovo RNAi caused eyes to gradually shrink until they were completely absent. One hundred percent of ovo RNAi-treated planarians lost both eyes within 137 days of treatment onset. ovo RNAi-treated planarians were unable to regenerate eyes in response to decapitation. Upon removal of ovo RNAi, eyes became visible as small pigmented spots in the head within 28 days. The eyes slowly developed, appearing to gain pigmented cells first and then nonpigmented photoreceptors. Phototaxis assays demonstrated functional eye loss and eye restoration. ovo mRNA was significantly decreased following treatment with ovo RNAi and significantly increased following removal of ovo RNAi. Arrestin staining was present in the eyes, optic nerves, and optic chiasm of worms with regenerated eyes but not in eyeless worms. CONCLUSIONS. S. mediterranea have the ability to generate functional eyes in the absence of a wound healing response. This ability requires the expression of ovo.

KW - Ovo

KW - Phototaxis

KW - Planarian

KW - Regeneration

KW - Schmidtea mediterranea

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