Contributions of microbiome and mechanical deformation to intestinal bacterial overgrowth and inflammation in a human gut-on-a-chip

Hyun Jung Kim, Hu Li, James J. Collins, Donald E. Ingber

Research output: Contribution to journalArticle

227 Citations (Scopus)

Abstract

A human gut-on-a-chip microdevice was used to coculture multiple commensal microbes in contact with living human intestinal epithelial cells formore than a week in vitro and to analyze how gut microbiome, inflammatory cells, and peristalsis-associated mechanical deformations independently contribute to intestinal bacterial overgrowth and inflammation. This in vitro model replicated results from past animal and human studies, including demonstration that probiotic and antibiotic therapies can suppress villus injury induced by pathogenic bacteria. By ceasing peristalsis-like motions while maintaining luminal flow, lack of epithelial deformation was shown to trigger bacterial overgrowth similar to that observed in patients with ileus and inflammatory bowel disease. Analysis of intestinal inflammation on-chip revealed that immune cells and lipopolysaccharide endotoxin together stimulate epithelial cells to produce four proinflammatory cytokines (IL-8, IL-6, IL-1β, and TNF-α) that are necessary and sufficient to induce villus injury and compromise intestinal barrier function. Thus, this human gut-on-a-chip can be used to analyze contributions of microbiome to intestinal pathophysiology and dissect disease mechanisms in a controlled manner that is not possible using existing in vitro systems or animal models.

Original languageEnglish (US)
Pages (from-to)E7-E15
JournalProceedings of the National Academy of Sciences of the United States of America
Volume113
Issue number1
DOIs
StatePublished - Jan 5 2016

Fingerprint

Microbiota
Inflammation
Peristalsis
Epithelial Cells
Ileus
Wounds and Injuries
Probiotics
Coculture Techniques
Interleukin-8
Interleukin-1
Inflammatory Bowel Diseases
Endotoxins
Lipopolysaccharides
Interleukin-6
Animal Models
Cytokines
Anti-Bacterial Agents
Bacteria
In Vitro Techniques
Gastrointestinal Microbiome

Keywords

  • Gut-on-a-chip
  • Inflammatory bowel disease
  • Intestine
  • Mechanical
  • Microbiome

ASJC Scopus subject areas

  • General

Cite this

Contributions of microbiome and mechanical deformation to intestinal bacterial overgrowth and inflammation in a human gut-on-a-chip. / Kim, Hyun Jung; Li, Hu; Collins, James J.; Ingber, Donald E.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 113, No. 1, 05.01.2016, p. E7-E15.

Research output: Contribution to journalArticle

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