Contribution of very low-density lipoprotein triglyceride fatty acids to postabsorptive free fatty acid flux in obese humans

Nikki C. Bush, Jessica M. Triay, Nicola W. Gathaiya, Kazanna C. Hames, Michael D. Jensen

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Objective In the fasting state, plasma free fatty acids (FFA) are thought to derive almost exclusively from adipose tissue lipolysis. However, there are mixed reports as to whether the spillover of fatty acids (FA) from very low-density lipoprotein triglyceride (VLDL-TG) hydrolysis contributes significantly to the plasma FFA pool. Because substantial VLDL-TG fatty acid spillover into the plasma FFA pool would profoundly impact the interpretation of isotope dilution measures of FFA flux, we investigated the contribution of VLDL-TG spillover to plasma FFA appearance. Materials/Methods Eighteen obese adults (15 women) participated in these studies. Each volunteer received a primed, continuous infusion of their own ex-vivo labeled ([1- 14C]triolein) VLDL-TG and a continuous infusion of [U- 13C]oleate (8 nmol·kg fat free mass- 1· min- 1) to measure VLDL-TG and FFA rate of appearance (Ra), respectively. The presence of 14C-oleate in the plasma FFA-oleate pool was used to calculate the contribution of spillover from VLDL-TG-oleate to the plasma FFA-oleate Ra. Results The spillover rate of VLDL-TG-oleate into plasma FFA-oleate was 6 ± 2 μmol/min (7% ± 2% of [ 14C]oleate from VLDL-TG) and FFA-oleate flux was 240 ± 61 μmol/min. Thus, only 3% ± 1% of total plasma FFA-oleate appearance could be accounted for by VLDL-TG spillover. Conclusion The contribution of VLDL-TG spillover to the total plasma FFA pool is negligible and will not materially affect the interpretation of FFA flux measures as an index of adipose tissue lipolysis.

Original languageEnglish (US)
Pages (from-to)137-140
Number of pages4
JournalMetabolism: Clinical and Experimental
Volume63
Issue number1
DOIs
StatePublished - Jan 2014

Keywords

  • Hypertriglyceridemia
  • Isotope dilution
  • [C]triolein
  • [U-C]oleate

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

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