Contribution of nitric oxide in the contraction-induced rapid vasodilation in young and older adults

Darren P. Casey, Branton G. Walker, Sushant M. Ranadive, Jennifer L. Taylor, Michael Joseph Joyner

Research output: Contribution to journalArticle

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Abstract

Contribution of nitric oxide in the contraction-induced rapid vasodilation in young and older adults. J Appl Physiol 115: 446-455, 2013. First published June 20, 2013; doi:10.1152/japplphysiol.00446.2013.-We tested the hypothesis that reduced nitric oxide (NO) bioavailability contributes to the attenuated peak and total vasodilation following singlemuscle contractions in older adults. Young (n = 10; 24 ± 2 yr) and older (n = 10; 67 ± 2 yr) adults performed single forearm contractions at 10, 20, and 40% of maximum during saline infusion (control) and NO synthase (NOS) inhibition via NG-monomethyl-L-arginine. Brachial artery diameters and velocities were measured using Doppler ultrasound and forearm vascular conductance (FVC; in ml.min 1.100 mmHg-1) was calculated from blood flow (ml/min) and blood pressure (mmHg). Peak and total vasodilator responses [change (δ) in FVC from baseline] were attenuated in older adults at all intensities (P < 0.05). NOS inhibition reduced the peak δFVC at 10% (88 ± 12 vs. 52 ± 9 ml.min-1.100 mmHg-1), 20% (125 ± 13 vs. 83 ± 13 ml.min-1.100 mmHg-1), and 40% (207 ± 26 vs. 133 ± 20 ml.min-1.100 mmHg-1) in young subjects, (P < 0.05 for all) and in older adults at 10% (59 ± 5 vs. 47±7 ml.min-1.100 mmHg-1, P<0.05) and 20% (88±9 vs. 68± 9 ml.min-1.100 mmHg-1, P < 0.05), but not 40% (128 ± 12 vs. 105 ± 11 ml.min-1.100 mmHg -1, P = 0.11). The relative (%) reduction in peak δFVC due to NOS inhibition was greater in young vs. older adults at 20% (-36 ± 5 vs. -23 ± 5%, P < 0.05) and 40% (-35 ± 6 vs. -16 ± 7%, P < 0.05). The reduction in the total vasodilator response (area under the curve) with NOS inhibition was also greater in young vs. older adults at all intensities. Our data suggest that contraction-induced rapid vasodilation is mediated in part by NO, and that the contribution of NO is greater in young adults.

Original languageEnglish (US)
Pages (from-to)446-455
Number of pages10
JournalJournal of Applied Physiology
Volume115
Issue number4
DOIs
StatePublished - Aug 15 2013

Fingerprint

Vasodilation
Nitric Oxide Synthase
Young Adult
Nitric Oxide
Vasodilator Agents
Forearm
omega-N-Methylarginine
Doppler Ultrasonography
Brachial Artery
Biological Availability
Area Under Curve
Blood Vessels
Blood Pressure

Keywords

  • Aging
  • Muscle contraction
  • Nitric oxide
  • Vasodilation

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Contribution of nitric oxide in the contraction-induced rapid vasodilation in young and older adults. / Casey, Darren P.; Walker, Branton G.; Ranadive, Sushant M.; Taylor, Jennifer L.; Joyner, Michael Joseph.

In: Journal of Applied Physiology, Vol. 115, No. 4, 15.08.2013, p. 446-455.

Research output: Contribution to journalArticle

Casey, Darren P. ; Walker, Branton G. ; Ranadive, Sushant M. ; Taylor, Jennifer L. ; Joyner, Michael Joseph. / Contribution of nitric oxide in the contraction-induced rapid vasodilation in young and older adults. In: Journal of Applied Physiology. 2013 ; Vol. 115, No. 4. pp. 446-455.
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