Contrasting roles for CD4 vs. CD8 T-cells in a murine model of virally induced "T1 black hole" formation

Istvan Pirko, Yi Chen, Anne K. Lohrey, Jeremiah McDole, Jeffrey D. Gamez, Kathleen S. Allen, Kevin D. Pavelko, Diana M. Lindquist, R. Scott Dunn, Slobodan I Macura, Aaron J. Johnson

Research output: Contribution to journalArticle

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Abstract

MRI is sensitive to tissue pathology in multiple sclerosis (MS); however, most lesional MRI findings have limited correlation with disability. Chronic T1 hypointense lesions or "T1 black holes" (T1BH), observed in a subset of MS patients and thought to represent axonal damage, show moderate to strong correlation with disability. The pathogenesis of T1BH remains unclear. We previously reported the first and as of yet only model of T1BH formation in the Theiler's murine encephalitis virus induced model of acute CNS neuroinflammation induced injury, where CD8 T-cells are critical mediators of axonal damage and related T1BH formation. The purpose of this study was to further analyze the role of CD8 and CD4 T-cells through adoptive transfer experiments and to determine if the relevant CD8 T-cells are classic epitope specific lymphocytes or different subsets. C57BL/6 mice were used as donors and RAG-1 deficient mice as hosts in our adoptive transfer experiments. In vivo 3-dimensional MRI images were acquired using a 7 Tesla small animal MRI system. For image analysis, we used semi-automated methods in Analyze 9.1; transfer efficiency was monitored using FACS of brain infiltrating lymphocytes. Using a peptide depletion method, we demonstrated that the majority of CD8 T-cells are classic epitope specific cytotoxic cells. CD8 T-cell transfer successfully restored the immune system's capability to mediate T1BH formation in animals that lack adaptive immune system, whereas CD4 T-cell transfer results in an attenuated phenotype with significantly less T1BH formation. These findings demonstrate contrasting roles for these cell types, with additional evidence for a direct pathogenic role of CD8 T-cells in our model of T1 black hole formation.

Original languageEnglish (US)
Article numbere31459
JournalPLoS One
Volume7
Issue number2
DOIs
StatePublished - Feb 13 2012

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T-cells
T-lymphocytes
animal models
T-Lymphocytes
Magnetic resonance imaging
T-Lymphocyte Epitopes
Adoptive Transfer
Multiple Sclerosis
Immune System
Lymphocytes
Immune system
sclerosis
Encephalitis Viruses
epitopes
Epitopes
mice
Animals
lymphocytes
Inbred C57BL Mouse
Pathology

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Contrasting roles for CD4 vs. CD8 T-cells in a murine model of virally induced "T1 black hole" formation. / Pirko, Istvan; Chen, Yi; Lohrey, Anne K.; McDole, Jeremiah; Gamez, Jeffrey D.; Allen, Kathleen S.; Pavelko, Kevin D.; Lindquist, Diana M.; Dunn, R. Scott; Macura, Slobodan I; Johnson, Aaron J.

In: PLoS One, Vol. 7, No. 2, e31459, 13.02.2012.

Research output: Contribution to journalArticle

Pirko, I, Chen, Y, Lohrey, AK, McDole, J, Gamez, JD, Allen, KS, Pavelko, KD, Lindquist, DM, Dunn, RS, Macura, SI & Johnson, AJ 2012, 'Contrasting roles for CD4 vs. CD8 T-cells in a murine model of virally induced "T1 black hole" formation', PLoS One, vol. 7, no. 2, e31459. https://doi.org/10.1371/journal.pone.0031459
Pirko, Istvan ; Chen, Yi ; Lohrey, Anne K. ; McDole, Jeremiah ; Gamez, Jeffrey D. ; Allen, Kathleen S. ; Pavelko, Kevin D. ; Lindquist, Diana M. ; Dunn, R. Scott ; Macura, Slobodan I ; Johnson, Aaron J. / Contrasting roles for CD4 vs. CD8 T-cells in a murine model of virally induced "T1 black hole" formation. In: PLoS One. 2012 ; Vol. 7, No. 2.
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