Contrast nephrotoxicity: Predictive value of urinary enzyme markers in a rat model

Sailendra Naidu, Fred T. Lee

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Rationale and Objectives. We tested whether urinary enzymes are an accurate and useful marker of renal damage in a rat model of contrast media nephrotoxicity. Methods.: Thirty rats were pretreated with a combination of salt depletion, indomethacin, and contrast material. Alanine aminopeptidase (AAP), γ-glutamyl-transpeptidase (GGT), and N-acetyl-β-d-glucosaminidase (NAG) were measured before and 24 hr after injection of contrast material. Enzyme concentrations were correlated with glomerular filtration rate (GFR) and histology. Results.: Decreasing GFR and histopathologic changes were found only in rats treated with all three variables. NAG levels increased from baseline for both diatrizoate meglumine- and ioversol-treated animals (from 83.9±48.6 to 145.5±55.4 and from 69.41±43.6 to 123.1±50.7 respectively; P<0.05 from baseline for ioversol) and declined in other groups. GGT and AAP levels did not correlate well with structural and functional changes. Conclusions.: In this model of contrast nephrotoxicity, NAG concentration appears to correlate with structural and functional changes associated with contrast media nephrotoxicity. However, the large range of baseline values makes this of dubious clinical use. AAP and GGT levels appear less helpful in detecting renal damage.

Original languageEnglish (US)
Pages (from-to)3-9
Number of pages7
JournalAcademic Radiology
Volume1
Issue number1
DOIs
StatePublished - Jan 1 1994
Externally publishedYes

Fingerprint

ioversol
CD13 Antigens
Hexosaminidases
Contrast Media
Enzymes
Glomerular Filtration Rate
Diatrizoate Meglumine
Kidney
gamma-Glutamyltransferase
Indomethacin
Histology
Salts
Injections

Keywords

  • contrast media, effects
  • contrast media, experimental studies
  • Contrast media, toxicity
  • diatrizoate
  • enzymes
  • ioversol

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Cite this

Contrast nephrotoxicity : Predictive value of urinary enzyme markers in a rat model. / Naidu, Sailendra; Lee, Fred T.

In: Academic Radiology, Vol. 1, No. 1, 01.01.1994, p. 3-9.

Research output: Contribution to journalArticle

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abstract = "Rationale and Objectives. We tested whether urinary enzymes are an accurate and useful marker of renal damage in a rat model of contrast media nephrotoxicity. Methods.: Thirty rats were pretreated with a combination of salt depletion, indomethacin, and contrast material. Alanine aminopeptidase (AAP), γ-glutamyl-transpeptidase (GGT), and N-acetyl-β-d-glucosaminidase (NAG) were measured before and 24 hr after injection of contrast material. Enzyme concentrations were correlated with glomerular filtration rate (GFR) and histology. Results.: Decreasing GFR and histopathologic changes were found only in rats treated with all three variables. NAG levels increased from baseline for both diatrizoate meglumine- and ioversol-treated animals (from 83.9±48.6 to 145.5±55.4 and from 69.41±43.6 to 123.1±50.7 respectively; P<0.05 from baseline for ioversol) and declined in other groups. GGT and AAP levels did not correlate well with structural and functional changes. Conclusions.: In this model of contrast nephrotoxicity, NAG concentration appears to correlate with structural and functional changes associated with contrast media nephrotoxicity. However, the large range of baseline values makes this of dubious clinical use. AAP and GGT levels appear less helpful in detecting renal damage.",
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