Contrast in cytokine expression between patients with monoclonal gammopathy of undetermined significance or multiple myeloma

K. A. Donovan, M. Q. Lacy, M. P. Kline, G. J. Ahmann, J. K. Heimbach, R. A. Kyle, J. A. Lust

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

We investigated whether differences in IL-6 and IL-1β expression could be detected in monoclonal plasma cells from patients with MGUS or MM. Expression of IL-6 and IL-1β in bone marrow cells was determined using cell sorting to enrich for plasma cells followed by reverse transcriptase/polymerase chain reaction (RT/PCR). Nineteen patients (six MGUS, two primary amyloid (AL), 11 MM) were studied. IL-6 mRNA expression was detectable in the sorted CD38+/CD45- plasma cell populations from 0/6 MGUS, 0/2 AL and 5/11 MM patients. All five MM patients with autocrine IL-6 expression demonstrated an elevated plasma cell labeling index. IL-1β mRNA was detectable in the sorted CD38+/CD45- plasma cell populations from 1/6 MGUS, 0/2 AL and 10/11 MM patients. In situ hybridization (ISH) confirmed that the IL-1β producing cells were plasma cells. In conclusion, autocrine production of IL-6 parallels a high labeling index and aberrant expression of IL-1β correlates with the diagnosis of MM. Follow-up of IL-1β-positive MGUS patients will determine whether aberrant expression of IL-1β will predict those MGUS patients that will eventually progress to MM.

Original languageEnglish (US)
Pages (from-to)593-600
Number of pages8
JournalLeukemia
Volume12
Issue number4
DOIs
StatePublished - Jan 1 1998

Keywords

  • Interleukin-1β
  • Interleukin-6
  • MGUS
  • Myeloma
  • RT/PCR

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

Fingerprint Dive into the research topics of 'Contrast in cytokine expression between patients with monoclonal gammopathy of undetermined significance or multiple myeloma'. Together they form a unique fingerprint.

  • Cite this