TY - JOUR
T1 - Contractile Activity of Circular Smooth Muscle in Rats One Year after Small Bowel Transplantation
T2 - Differing Adaptive Response of the Jejunum and Ileum to Denervation
AU - Shibata, Chikashi
AU - Murr, Michel M.
AU - Balsiger, Bruno
AU - Anding, William J.
AU - Sarr, Michael G.
N1 - Funding Information:
From the Department of Surgery and the GastroenterologyR esearch Umt, Mayo Chmc and Mayo Foundauon, Rochester,M ann Supported by grant DK 39337 from the Nauonal Instatuteso f Health, Umted States Pubhc Health Serwce (M G S.), and by Educon Cor-porauon, the Mayo Foundauon, and the First Department of Surgery,T ohokuU mversltyS chool of Me&cane Parts of flus studyw ere presented at the annual meetingo f the American GastroenterologlcalA ssooauon, San Francisco, Callf, 1996, and the Thirty-Eighth Annual Meeting of The Societyf or Surgery of the AlimentaryT ract, Washmgton, D C, May 11-14, 1997 Abstracts of this work were pubhshed m Gastroenterology1 10 A758, 1996, and Gastroenterology1 12 A824, 1997 Correspondence Machael G Sarr, M D, Professor of Surgery, GastroenterologyR esearch Unit, Mayo Clnuc, 200 First Street SW, Rochester, MN 55905
PY - 1998
Y1 - 1998
N2 - The aim of the present study was to determine the long-term effects of isogeneic small bowel transplantation (SBT) on jejunal and ileal circular smooth muscle contractile activity in the rat. Transmural strips of circular muscle were prepared from proximal jejunum and distal ileum of 1-year-old control rats and rats 1 year after SBT (SBT-1Y) to measure isometric force. Spontaneous contractile activity and the dose-responses to bethanechol and norepinephrine were studied Electrical field stimulation (EFS) at varying frequencies (1 to 20 Hz) was evaluated under adrenergic and cholinergic blockade to investigate inhibitory nerves Spontaneous activity both in the jejunum and ileum in SBT-1Y rats was not different compared to control rats Sensitivity to bethanechol did not differ between control and SBT-1Y rats in the jejunum or ileum Sensitivity to norepinephrine, however, was significantly increased after SBT in the ileum but not in the jejunum During EFS, inhibition was seen at low frequencies, and contractions were induced at high frequencies in all groups The degree of inhibition did not differ between control and SBT-1Y rats in the jejunum, however, it tended to be increased in the ileum after SBT The long-term adaptive response of smooth muscle to the extrinsic denervation accompanying SBT differs between the jejunum and the ileum.
AB - The aim of the present study was to determine the long-term effects of isogeneic small bowel transplantation (SBT) on jejunal and ileal circular smooth muscle contractile activity in the rat. Transmural strips of circular muscle were prepared from proximal jejunum and distal ileum of 1-year-old control rats and rats 1 year after SBT (SBT-1Y) to measure isometric force. Spontaneous contractile activity and the dose-responses to bethanechol and norepinephrine were studied Electrical field stimulation (EFS) at varying frequencies (1 to 20 Hz) was evaluated under adrenergic and cholinergic blockade to investigate inhibitory nerves Spontaneous activity both in the jejunum and ileum in SBT-1Y rats was not different compared to control rats Sensitivity to bethanechol did not differ between control and SBT-1Y rats in the jejunum or ileum Sensitivity to norepinephrine, however, was significantly increased after SBT in the ileum but not in the jejunum During EFS, inhibition was seen at low frequencies, and contractions were induced at high frequencies in all groups The degree of inhibition did not differ between control and SBT-1Y rats in the jejunum, however, it tended to be increased in the ileum after SBT The long-term adaptive response of smooth muscle to the extrinsic denervation accompanying SBT differs between the jejunum and the ileum.
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U2 - 10.1016/S1091-255X(98)80038-3
DO - 10.1016/S1091-255X(98)80038-3
M3 - Article
C2 - 9843607
AN - SCOPUS:0032149945
SN - 1091-255X
VL - 2
SP - 463
EP - 472
JO - Journal of Gastrointestinal Surgery
JF - Journal of Gastrointestinal Surgery
IS - 5
ER -