Construction of a transcription map surrounding the BRCA1 locus of human chromosome 17

Lawrence C. Brody; Kenneth J. Abel; Lucio H. Castilla; Fergus J. Couch; Dawn R. McKinley; Guiying Yin; Peggy P. Ho; Sofia Merajver; Settara C. Chandrasekharappa; Junzhe Xu; Jeffery L. Cole; Jeffery P. Struewing; John M. Valdes; Francis S. Collins; Barbara L. Weber

doi:10.1016/0888-7543(95)80131-5

Construction of a transcription map surrounding the BRCA1 locus of human chromosome 17

Lawrence C. Brody, Kenneth J. Abel, Lucio H. Castilla, Fergus J. Couch, Dawn R. McKinley, Guiying Yin, Peggy P. Ho, Sofia Merajver, Settara C. Chandrasekharappa, Junzhe Xu, Jeffery L. Cole, Jeffery P. Struewing, John M. Valdes, Francis S. Collins, Barbara L. Weber

Research output: Contribution to journal › Article › peer-review

56 Scopus citations

Abstract

We have used a combination of methods (exon amplification, direct selection, direct screening, evolutionary conservation, island rescue-PCR, and direct sequence analysis) to survey approximately 600 kb of genomic DNA surrounding the BRCA1 gene for transcribed sequences. We have cloned a set of fragments representing at least 26 genes. The DNA sequence of these clones reveals that 5 are previously cloned genes; the precise chromosomal location of 2 was previously unknown, and 3 have been cloned and mapped by others to this interval. Three other genes, including BRCA1 itself, have recently been mapped independently to this region. Sequences from 11 genes are similar but not identical matches to known genes; 5 of these appear to be the human homologues of genes cloned from other species. Another 7 genes have no similarity with known genes. In addition, 39 putative exons and 14 expressed sequence tags have been identified and mapped to individual cosmids. This transcript map provides a detailed description of gene organization for this region of the genome.

Original language	English (US)
Pages (from-to)	238-247
Number of pages	10
Journal	Genomics
Volume	25
Issue number	1
DOIs	https://doi.org/10.1016/0888-7543(95)80131-5
State	Published - Jan 1 1995

ASJC Scopus subject areas

Genetics

Access to Document

10.1016/0888-7543(95)80131-5

Cite this

Brody, L. C., Abel, K. J., Castilla, L. H., Couch, F. J., McKinley, D. R., Yin, G., Ho, P. P., Merajver, S., Chandrasekharappa, S. C., Xu, J., Cole, J. L., Struewing, J. P., Valdes, J. M., Collins, F. S., & Weber, B. L. (1995). Construction of a transcription map surrounding the BRCA1 locus of human chromosome 17. Genomics, 25(1), 238-247. https://doi.org/10.1016/0888-7543(95)80131-5

@article{6386b93ecda3427490cea257cca03ede,

title = "Construction of a transcription map surrounding the BRCA1 locus of human chromosome 17",

abstract = "We have used a combination of methods (exon amplification, direct selection, direct screening, evolutionary conservation, island rescue-PCR, and direct sequence analysis) to survey approximately 600 kb of genomic DNA surrounding the BRCA1 gene for transcribed sequences. We have cloned a set of fragments representing at least 26 genes. The DNA sequence of these clones reveals that 5 are previously cloned genes; the precise chromosomal location of 2 was previously unknown, and 3 have been cloned and mapped by others to this interval. Three other genes, including BRCA1 itself, have recently been mapped independently to this region. Sequences from 11 genes are similar but not identical matches to known genes; 5 of these appear to be the human homologues of genes cloned from other species. Another 7 genes have no similarity with known genes. In addition, 39 putative exons and 14 expressed sequence tags have been identified and mapped to individual cosmids. This transcript map provides a detailed description of gene organization for this region of the genome.",

author = "Brody, {Lawrence C.} and Abel, {Kenneth J.} and Castilla, {Lucio H.} and Couch, {Fergus J.} and McKinley, {Dawn R.} and Guiying Yin and Ho, {Peggy P.} and Sofia Merajver and Chandrasekharappa, {Settara C.} and Junzhe Xu and Cole, {Jeffery L.} and Struewing, {Jeffery P.} and Valdes, {John M.} and Collins, {Francis S.} and Weber, {Barbara L.}",

note = "Funding Information: The authors thank Joan Effiom for assistance in the preparation of the manuscript and Sonye Danoff for critical reading and helpful suggestions. This work was supported by NIH Grant CA-57601 (B.W.).",

year = "1995",

month = jan,

day = "1",

doi = "10.1016/0888-7543(95)80131-5",

language = "English (US)",

volume = "25",

pages = "238--247",

journal = "Genomics",

issn = "0888-7543",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - Construction of a transcription map surrounding the BRCA1 locus of human chromosome 17

AU - Brody, Lawrence C.

AU - Abel, Kenneth J.

AU - Castilla, Lucio H.

AU - Couch, Fergus J.

AU - McKinley, Dawn R.

AU - Yin, Guiying

AU - Ho, Peggy P.

AU - Merajver, Sofia

AU - Chandrasekharappa, Settara C.

AU - Xu, Junzhe

AU - Cole, Jeffery L.

AU - Struewing, Jeffery P.

AU - Valdes, John M.

AU - Collins, Francis S.

AU - Weber, Barbara L.

N1 - Funding Information: The authors thank Joan Effiom for assistance in the preparation of the manuscript and Sonye Danoff for critical reading and helpful suggestions. This work was supported by NIH Grant CA-57601 (B.W.).

PY - 1995/1/1

Y1 - 1995/1/1

N2 - We have used a combination of methods (exon amplification, direct selection, direct screening, evolutionary conservation, island rescue-PCR, and direct sequence analysis) to survey approximately 600 kb of genomic DNA surrounding the BRCA1 gene for transcribed sequences. We have cloned a set of fragments representing at least 26 genes. The DNA sequence of these clones reveals that 5 are previously cloned genes; the precise chromosomal location of 2 was previously unknown, and 3 have been cloned and mapped by others to this interval. Three other genes, including BRCA1 itself, have recently been mapped independently to this region. Sequences from 11 genes are similar but not identical matches to known genes; 5 of these appear to be the human homologues of genes cloned from other species. Another 7 genes have no similarity with known genes. In addition, 39 putative exons and 14 expressed sequence tags have been identified and mapped to individual cosmids. This transcript map provides a detailed description of gene organization for this region of the genome.

AB - We have used a combination of methods (exon amplification, direct selection, direct screening, evolutionary conservation, island rescue-PCR, and direct sequence analysis) to survey approximately 600 kb of genomic DNA surrounding the BRCA1 gene for transcribed sequences. We have cloned a set of fragments representing at least 26 genes. The DNA sequence of these clones reveals that 5 are previously cloned genes; the precise chromosomal location of 2 was previously unknown, and 3 have been cloned and mapped by others to this interval. Three other genes, including BRCA1 itself, have recently been mapped independently to this region. Sequences from 11 genes are similar but not identical matches to known genes; 5 of these appear to be the human homologues of genes cloned from other species. Another 7 genes have no similarity with known genes. In addition, 39 putative exons and 14 expressed sequence tags have been identified and mapped to individual cosmids. This transcript map provides a detailed description of gene organization for this region of the genome.

UR - http://www.scopus.com/inward/record.url?scp=0028947911&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028947911&partnerID=8YFLogxK

U2 - 10.1016/0888-7543(95)80131-5

DO - 10.1016/0888-7543(95)80131-5

M3 - Article

C2 - 7774924

AN - SCOPUS:0028947911

SN - 0888-7543

VL - 25

SP - 238

EP - 247

JO - Genomics

JF - Genomics

IS - 1

ER -

Construction of a transcription map surrounding the BRCA1 locus of human chromosome 17

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this