Construction of a recombinant feline herpesvirus type 1 expressing Gag precursor protein of feline immunodeficiency virus

E. Sato, N. Yokoyama, K. Maeda, Y. Inoshima, M. Kohmoto, Yasuhiro H Ikeda, T. Miyazawa, T. Mikami

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

We constructed a deletion mutant of feline herpesvirus type 1 (FHV-1) and a recombinant FHV-1. The deletion mutant is the virus with a region (367 bp) deleted from the start codon of thymidine kinase (TK) gene to the SmaI site within the TK gene, and the other is a recombinant FHV-1 expressing Gag protein of feline immunodeficiency virus (FIV), in which a cDNA encoding the Gag protein of FIV was inserted at the TK deletion site of the former deletion mutant. These viruses were designated as C7301ddlTK and C730lddlTK-gag, respectively. Growth kinetics of these viruses in Crandell feline kidney cells was similar to that of the parent C7301 strain. By immunoblot analysis, C730lddlTK-gag was confirmed to express the FIV Gag precursor protein in the cells.

Original languageEnglish (US)
Pages (from-to)453-466
Number of pages14
JournalArchives of Virology
Volume143
Issue number3
DOIs
StatePublished - 1998
Externally publishedYes

Fingerprint

Feline Immunodeficiency Virus
gag Gene Products
Herpesviridae
Felidae
Thymidine Kinase
Viruses
Initiator Codon
Genes
Complementary DNA
Kidney
Growth

ASJC Scopus subject areas

  • Genetics
  • Applied Microbiology and Biotechnology

Cite this

Sato, E., Yokoyama, N., Maeda, K., Inoshima, Y., Kohmoto, M., Ikeda, Y. H., ... Mikami, T. (1998). Construction of a recombinant feline herpesvirus type 1 expressing Gag precursor protein of feline immunodeficiency virus. Archives of Virology, 143(3), 453-466. https://doi.org/10.1007/s007050050302

Construction of a recombinant feline herpesvirus type 1 expressing Gag precursor protein of feline immunodeficiency virus. / Sato, E.; Yokoyama, N.; Maeda, K.; Inoshima, Y.; Kohmoto, M.; Ikeda, Yasuhiro H; Miyazawa, T.; Mikami, T.

In: Archives of Virology, Vol. 143, No. 3, 1998, p. 453-466.

Research output: Contribution to journalArticle

Sato, E, Yokoyama, N, Maeda, K, Inoshima, Y, Kohmoto, M, Ikeda, YH, Miyazawa, T & Mikami, T 1998, 'Construction of a recombinant feline herpesvirus type 1 expressing Gag precursor protein of feline immunodeficiency virus', Archives of Virology, vol. 143, no. 3, pp. 453-466. https://doi.org/10.1007/s007050050302
Sato, E. ; Yokoyama, N. ; Maeda, K. ; Inoshima, Y. ; Kohmoto, M. ; Ikeda, Yasuhiro H ; Miyazawa, T. ; Mikami, T. / Construction of a recombinant feline herpesvirus type 1 expressing Gag precursor protein of feline immunodeficiency virus. In: Archives of Virology. 1998 ; Vol. 143, No. 3. pp. 453-466.
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