Construction of a recombinant feline herpesvirus type 1 expressing Gag precursor protein of feline immunodeficiency virus

E. Sato, N. Yokoyama, K. Maeda, Y. Inoshima, M. Kohmoto, Y. Ikeda, T. Miyazawa, T. Mikami

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

We constructed a deletion mutant of feline herpesvirus type 1 (FHV-1) and a recombinant FHV-1. The deletion mutant is the virus with a region (367 bp) deleted from the start codon of thymidine kinase (TK) gene to the SmaI site within the TK gene, and the other is a recombinant FHV-1 expressing Gag protein of feline immunodeficiency virus (FIV), in which a cDNA encoding the Gag protein of FIV was inserted at the TK deletion site of the former deletion mutant. These viruses were designated as C7301ddlTK and C730lddlTK-gag, respectively. Growth kinetics of these viruses in Crandell feline kidney cells was similar to that of the parent C7301 strain. By immunoblot analysis, C730lddlTK-gag was confirmed to express the FIV Gag precursor protein in the cells.

Original languageEnglish (US)
Pages (from-to)453-466
Number of pages14
JournalArchives of Virology
Volume143
Issue number3
DOIs
StatePublished - Apr 27 1998

ASJC Scopus subject areas

  • Virology

Fingerprint Dive into the research topics of 'Construction of a recombinant feline herpesvirus type 1 expressing Gag precursor protein of feline immunodeficiency virus'. Together they form a unique fingerprint.

  • Cite this

    Sato, E., Yokoyama, N., Maeda, K., Inoshima, Y., Kohmoto, M., Ikeda, Y., Miyazawa, T., & Mikami, T. (1998). Construction of a recombinant feline herpesvirus type 1 expressing Gag precursor protein of feline immunodeficiency virus. Archives of Virology, 143(3), 453-466. https://doi.org/10.1007/s007050050302